Anti-Hepatitis C Virus Core Antigen antibody (ab58713)
Key features and details
- Rabbit polyclonal to Hepatitis C Virus Core Antigen
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Isotype: IgG
Overview
-
Product name
Anti-Hepatitis C Virus Core Antigen antibody
See all Hepatitis C Virus Core Antigen primary antibodies -
Description
Rabbit polyclonal to Hepatitis C Virus Core Antigen -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Synthetic peptide corresponding to N-terminal residues of Hepatitis C virus core protein.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab58713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | 1/200. |
Target
-
Relevance
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients. -
Cellular localization
Endoplasmic reticulum -
Database links
- SwissProt: P26664 Hepatitis C virus
- SwissProt: P29846 Hepatitis C virus
-
Alternative names
- Core protein p21 antibody
- HCV core antigen antibody
- HCV core protein antibody
- Hepatitis C Virus core protein antibody
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus Core Antigen antibody (ab58713)This image is courtesy of an Abreview submitted by Nicole Corderab58713 staining Hepatitis C Virus Core Antigen in the Huh-7 hepatocyte cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.5% BSA) for 16 hours at 4°C. An FITC-conjugated goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.
Datasheets and documents
References
ab58713 has been referenced in 5 publications.
- Galasso M et al. Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion. Nat Commun 10:481 (2019). PubMed: 30696822
- Anticoli S et al. Counteraction of HCV-Induced Oxidative Stress Concurs to Establish Chronic Infection in Liver Cell Cultures. Oxid Med Cell Longev 2019:6452390 (2019). PubMed: 30906503
- Venkatesh AG et al. Clinical detection of Hepatitis C viral infection by yeast-secreted HCV-core:Gold-binding-peptide. Biosens Bioelectron 119:230-236 (2018). PubMed: 30144754
- Granato M et al. HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2. Sci Rep 6:30649 (2016). WB ; Human . PubMed: 27476557
- Smirnova OA et al. Hepatitis C Virus NS5A Protein Triggers Oxidative Stress by Inducing NADPH Oxidases 1 and 4 and Cytochrome P450 2E1. Oxid Med Cell Longev 2016:8341937 (2016). PubMed: 27200149
