Overview

  • Product name
    Anti-Hepatitis C Virus Core Antigen antibody
    See all Hepatitis C Virus Core Antigen primary antibodies
  • Description
    Rabbit polyclonal to Hepatitis C Virus Core Antigen
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of Hepatitis C virus core protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab58713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200.

Target

  • Relevance
    The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
  • Cellular localization
    Endoplasmic reticulum
  • Database links
    • Alternative names
      • Core protein p21 antibody
      • HCV core antigen antibody
      • HCV core protein antibody
      • Hepatitis C Virus core protein antibody

    Images

    • ab58713 staining Hepatitis C Virus Core Antigen in the Huh-7 hepatocyte cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.5% BSA) for 16 hours at 4°C. An FITC-conjugated goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Galasso M  et al. Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion. Nat Commun 10:481 (2019). Read more (PubMed: 30696822) »
    • Anticoli S  et al. Counteraction of HCV-Induced Oxidative Stress Concurs to Establish Chronic Infection in Liver Cell Cultures. Oxid Med Cell Longev 2019:6452390 (2019). Read more (PubMed: 30906503) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Answer

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