Product nameAnti-Hepatitis C Virus E2 antibody
See all Hepatitis C Virus E2 primary antibodies
DescriptionGoat polyclonal to Hepatitis C Virus E2
Tested applicationsSuitable for: ELISA, WB, Conjugationmore details
Species reactivityReacts with Hepatitis C Virus. Not yet tested in other species.
Recombinant full length E2 (genotype 1a) protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notes>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab20044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.|
|Conjugation||Use at an assay dependent dilution.|
RelevanceHepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
Cellular localizationViral envelope protein.
- Core protein antibody
- E1 protein antibody
- E2 protein antibody
ELISA analysis of Hepatitis C Virus E2 binding to CD81 receptor, using ab20044 to detect Hepatitis C Virus E2 binding.
A 96-well plate was coated with 100 ng of rho-1D4 antibody. Following overnight incubation at 4°C, the unbound antibody was washed away. The wells were blocked with blocking solution for 2 hours. 100 ng of purified CD81 was added to the wells along with E2 glycoprotein (0.4 to 20 nM) and incubated for 2 hours. ab20044 anti-Hepatitis C Virus E2 antibody (1/2000 dilution) and secondary antibody with alkaline phosphatase conjugate (1/1000 dilution) were added and incubated for 2 hours. Absorbance was measured at 495 nm by a plate-reader.
This product has been referenced in:
- Takayama H et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA ; Hepatitis C virus . Read more (PubMed: 18523555) »