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Anti-Hepatitis C Virus E2 antibody (ab20044)

Submit a review Q&A (3)References (1)
ELISA - Anti-Hepatitis C Virus E2 antibody (ab20044)

    Key features and details

    • Goat polyclonal to Hepatitis C Virus E2
    • Suitable for: ELISA, WB
    • Isotype: IgG

    Overview

    • Product name

      Anti-Hepatitis C Virus E2 antibody
      See all Hepatitis C Virus E2 primary antibodies

    • Description

      Goat polyclonal to Hepatitis C Virus E2

    • Host species

      Goat

    • Tested applications

      Suitable for: ELISA, WBmore details

    • Species reactivity

      Reacts with Hepatitis C Virus. Not yet tested in other species.

    • Immunogen

      Recombinant full length E2 (genotype 1a) protein.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: 0.0268% PBS
    • Concentration information loading...

    • Purity

      Ion Exchange Chromatography

    • Purification notes

      >95% pure. Sodium sulfate precipitation and ion-exchange chromatography.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab20044 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
    Notes
    ELISA
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.

    Target

    • Relevance

      Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.

    • Cellular localization

      Viral envelope protein.

    • Alternative names

      • Core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • Envelope glycoprotein E2 antibody
      • gp68 antibody
      • gp70 antibody
      • HCV E2 antibody
      • NS1 antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA dependent RNA polymerase antibody
      • P7 protein antibody
      • Polyprotein antibody
      see all

    Images

    • ELISA - Anti-Hepatitis C Virus E2 antibody (ab20044)
      ELISA - Anti-Hepatitis C Virus E2 antibody (ab20044)Image from Takayama H et al., PLoS One. 2008 Jun 4;3(6):e2314. doi: 10.1371/journal.pone.0002314.; Fig 5.; June 4, 2008, PLoS ONE 3(6): e2314.
      ELISA analysis of Hepatitis C Virus E2 binding to CD81 receptor, using ab20044 to detect Hepatitis C Virus E2 binding.

      A 96-well plate was coated with 100 ng of rho-1D4 antibody. Following overnight incubation at 4°C, the unbound antibody was washed away. The wells were blocked with blocking solution for 2 hours. 100 ng of purified CD81 was added to the wells along with E2 glycoprotein (0.4 to 20 nM) and incubated for 2 hours. ab20044 anti-Hepatitis C Virus E2 antibody (1/2000 dilution) and secondary antibody with alkaline phosphatase conjugate (1/1000 dilution) were added and incubated for 2 hours. Absorbance was measured at 495 nm by a plate-reader.

    References (1)

    Publishing research using ab20044? Please let us know so that we can cite the reference in this datasheet.

    ab20044 has been referenced in 1 publication.

    • Takayama H  et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA ; Hepatitis C virus . PubMed: 18523555

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    Product code: 20044

    Inquiry: Dear technical support team: The customer raised an enquiry about ab20044 (Anti-Hepatitis C Virus E2 antibody) and ab20852 (Anti-Hepatitis C Virus E2 antibody [BDI167]), he wants to know which one is more suitable for conduct the neutralization test? Could you please offer any selections or suggestions to him? Thanks for your kindly help Best regards, Ada

    Read More
    Answer

    Thank you for contacting us.

    ab20044 and ab20852 have not been tested in neutralisation experiment. We have no empirical data to support theuse in the application.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Hello,I am looking for antibodies (preferentially mouseMAb so I can use same secondary), reacting withvirions of hepatitis A virus (HAV), hepatitis C virus (HCV) and parvovirus B19. I have selected ab64295 forB19 and ab8254 for HAV. Could you, please confirm these would detect the intactvirions of B19 and HAV? Also, I am not sure which of your anti-HCV antibodies to choose for this purpose. Could you, please advice?

    Read More
    Answer

    Thank you for contacting us.

    Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience while waiting for the response.

    Concerning Parvovirus B19 antibody ab64295:
    Indeed, we can confirm that this antibody does detect the intact virus particles. This monoclonal antibody is specific for an epitope common to VP1 and VP2 structural proteins of human Parvovirus B19. These proteins do not need to be purified or the virus does not need to be disrupted in order for the antibody to recognize the target(s).

    Concerning Anti-Hepatitis A Virus antibody ab8254:
    The Hepatitis A pHM-175 strain was used as the immunogen. The clone MK-01 recognises hepatitis A virus surface antigen, unfortunately no further details are available if this antibody also detects intact virions. However, I am happy to let you know that we have ab1018 available which detects intact Hepatitis A virus:
    https://www.abcam.com/Hepatitis-A-Virus-antibody-ab1018.html
    https://www.abcam.com/Hepatitis-A-Virus-antibody-ab1018.html.

    Concerning Anti-Hepatitis C Virus antibodies: As the envelope glycoproteins, E1 and E2, are essential components of the HCV virion, I have looked for our antibodies against these targets.
    ab54555 and ab54556: Both antibodies can detect intact Hepatitis C virions. This has so far only been tested for the inactivated form, unfortunately not yet for non-inactivated virions.
    ab20044, ab20852, ab21306 : The target protein recognized by each antibody (HCV E1 or E2) are identified by testing on Western blot. These antibodies have also been tested by ELISA and IF/IHC, but these test methods do not identify if the epitopes are recognized on intact virions. It is therefore not known if these antibodies will recognize intact HCV virions.
    https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-ab54555.html https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-ab54555.html.
    https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab54556.html https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab54556.html.
    https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab20044.html https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-ab20044.html.
    https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-BDI167-ab20852.html https://www.abcam.com/Hepatitis-C-Virus-E2-antibody-BDI167-ab20852.html.
    https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-BDI198-ab21306.html https://www.abcam.com/Hepatitis-C-Virus-E1-antibody-BDI198-ab21306.html.

    Please be reassured that all our products are covered by our Abpromise: We will happily offer Scientific Support in the event that a product is not functioning in the applications and species cited on the product datasheet (and the problem has been reported within 6 months of purchase). If it appears that the antibody is faulty, a replacement, credit or refund will be offered (www.abcam.com/Abpromise).

    All tested applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program:

    As we do not currently have images for all these antibodies on our datasheets, I can offer a discount off a future purchase if you buy one or more of the products now, use it a tested application and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each.The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    Read More

    Question

    I have a question regarding immunogen for ab20044. As it is stated, the immunogen was 'Recombinant full lenght E2 (genotype 1a) protein'. Could you tell me, whether the immunogen was expressed in bacterial or eukaryotic system? I would like to know, if the antibody is able to recognize glycosylated form of E2 in western blot. Also, I would like to ask, if Donkey polyclonal to Goat IgG - H&L, Cy5, ab6566, is suitable for western blot applications.

    Read More
    Answer

    Thank you for your enquiry. I can confrim that the immunogen for ab20044 Hepatitis C Virus E2 antibody is a recombinant antigen expressed in CHO cells. This is a polyclonal antibody and therefore recognizes multiple epitopes on the E2 protein, but we have not tested this antibody for reactivity with glycosylated E2. With regards to the secondary antibody, ab6566 is not tested in Western Blot. All tested applications are listed on the datasheets which are updated as soon as we recieve any new information. I can recommend a more suitable secondary antibody to use in western blot with ab20044 Hepatitis C Virus E2 antibody is: ab6741 Rabbit polyclonal to Goat IgG - H&L (HRP) Tested in WB I can recommend reviewing the online datasheet for further information which I hope will be helpful to you: https://www.abcam.com/Rabbit-Goat-IgG-HL-HRP-ab6741.html I hope this information is useful to you. Should you have any further questions, please do not hesitate to contact us.

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