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I have a question regarding immunogen for ab20044. As it is stated, the immunogen was 'Recombinant full lenght E2 (genotype 1a) protein'. Could you tell me, whether the immunogen was expressed in bacterial or eukaryotic system? I would like to know, if the antibody is able to recognize glycosylated form of E2 in western blot. Also, I would like to ask, if Donkey polyclonal to Goat IgG - H&L, Cy5, ab6566, is suitable for western blot applications.
Asked on Nov 13 2008
Thank you for your enquiry. I can confrim that the immunogen for ab20044 Hepatitis C Virus E2 antibody is a recombinant antigen expressed in CHO cells. This is a polyclonal antibody and therefore recognizes multiple epitopes on the E2 protein, but we have not tested this antibody for reactivity with glycosylated E2. With regards to the secondary antibody, ab6566 is not tested in Western Blot. All tested applications are listed on the datasheets which are updated as soon as we recieve any new information. I can recommend a more suitable secondary antibody to use in western blot with ab20044 Hepatitis C Virus E2 antibody is: ab6741 Rabbit polyclonal to Goat IgG - H&L (HRP) Tested in WB I can recommend reviewing the online datasheet for further information which I hope will be helpful to you: https://www.abcam.com/Rabbit-Goat-IgG-HL-HRP-ab6741.html I hope this information is useful to you. Should you have any further questions, please do not hesitate to contact us.
Answered on Nov 13 2008