Overview

  • Product name
    Anti-Hepatitis C Virus E2 antibody
    See all Hepatitis C Virus E2 primary antibodies
  • Description
    Rabbit polyclonal to Hepatitis C Virus E2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Hepatitis C virus
  • Immunogen

    A synthetic peptide corresponding to N-terminal residues of Hepatitis C Virus E2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
  • Cellular localization
    Viral envelope protein.
  • Database links
    • Alternative names
      • Core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • Envelope glycoprotein E2 antibody
      • gp68 antibody
      • gp70 antibody
      • HCV E2 antibody
      • NS1 antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA dependent RNA polymerase antibody
      • P7 protein antibody
      • Polyprotein antibody
      see all

    References

    ab54556 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for contacting us.





    This antibody hs been tested against E2 protein from HCV genotype 1A. It works well



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for contacting us and your interest in our products.

    The immunogen sequence used to raise the anti-Hepatitis C Virus E2 antibody (ab54556) is proprietary information. However, in order to aid your research I am able to share it with you. Please do not share or publish this information. It is strictly for your own use.

    I can confirm that this antibody was raised against Hepatitis C Virus, genotype: 1B. It's immunogen corresponds to amino acids xxxxxx of Hepatitis C Virus E2.

    I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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    Answer

    Thank you for contacting us.

    Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience while waiting for the response.

    Concerning Parvovirus B19 antibody ab64295:
    Indeed, we can confirm that this antibody does detect the intact virus particles. This monoclonal antibody is specific for an epitope common to VP1 and VP2 structural proteins of human Parvovirus B19. These proteins do not need to be purified or the virus does not need to be disrupted in order for the antibody to recognize the target(s).

    Concerning Anti-Hepatitis A Virus antibody ab8254:
    The Hepatitis A pHM-175 strain was used as the immunogen. The clone MK-01 recognises hepatitis A virus surface antigen, unfortunately no further details are available if this antibody also detects intact virions. However, I am happy to let you know that we have ab1018 available which detects intact Hepatitis A virus:
    https://www.abcam.com/index.html?datasheet=1018
    https://www.abcam.com/index.html?datasheet=1018.

    Concerning Anti-Hepatitis C Virus antibodies: As the envelope glycoproteins, E1 and E2, are essential components of the HCV virion, I have looked for our antibodies against these targets.
    ab54555 and ab54556: Both antibodies can detect intact Hepatitis C virions. This has so far only been tested for the inactivated form, unfortunately not yet for non-inactivated virions.
    ab20044, ab20852, ab21306 : The target protein recognized by each antibody (HCV E1 or E2) are identified by testing on Western blot. These antibodies have also been tested by ELISA and IF/IHC, but these test methods do not identify if the epitopes are recognized on intact virions. It is therefore not known if these antibodies will recognize intact HCV virions.
    https://www.abcam.com/index.html?datasheet=54555 https://www.abcam.com/index.html?datasheet=54555.
    https://www.abcam.com/index.html?datasheet=54556 https://www.abcam.com/index.html?datasheet=54556.
    https://www.abcam.com/index.html?datasheet=20044 https://www.abcam.com/index.html?datasheet=20044.
    https://www.abcam.com/index.html?datasheet=20852 https://www.abcam.com/index.html?datasheet=20852.
    https://www.abcam.com/index.html?datasheet=21306 https://www.abcam.com/index.html?datasheet=21306.

    Please be reassured that all our products are covered by our Abpromise: We will happily offer Scientific Support in the event that a product is not functioning in the applications and species cited on the product datasheet (and the problem has been reported within 6 months of purchase). If it appears that the antibody is faulty, a replacement, credit or refund will be offered (www.abcam.com/Abpromise).

    All tested applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program:

    As we do not currently have images for all these antibodies on our datasheets, I can offer a discount off a future purchase if you buy one or more of the products now, use it a tested application and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each.The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

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    Answer

    Thank you for your inquiry. I can confirm that these antibodies are raised against Hepatitis C Virus, genotype: 1B. They were never testedwith genotype 2A and might therefore not be suitable for genotype 2A. I am sorry that I could not provide you with an positive answer on this occasion and hope this information is nevertheless helpful.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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