Overview

  • Product name
    Anti-Hepatitis C Virus genotype 1a NS5 antibody
  • Description
    Goat polyclonal to Hepatitis C Virus genotype 1a NS5
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with Hepatitis C Virus. Not yet tested in other species.
  • Immunogen

    Recombinant NS5 a&b genotype 1a (100% of a and 70% of b).

Properties

Applications

Our Abpromise guarantee covers the use of ab20773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. WB: 1/100. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV.
    • Cellular localization
      Endoplasmic reticulum membrane
    • Alternative names
      • HCV 1a NS5 antibody
      • Hepatitis C virus NS5A antibody
      • Non-structural protein 5A antibody
      • p56 antibody

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thanks for sending along your protocol.

    1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino acids 2212-2313 of Hepatitis C Virus NS5" The epitopes recognized by these antibodies would have to recognize NS5A within this small region to work.

    2. Usingponceau S stain post transfer, have you been able to determine protein is present on membrane after transfer?

    3. Regarding your protocol, a) Boiling of samplesis not recommended; instead heat at 70 deg C for 10min prior to loading gel b) Try blocking with 5% BSA instead of milk c) Use 1:1000 for primary antibodies and 1:5000 for secondary antibodies.

    I look forward to hearing if these suggestions prove useful.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Cell (huh 7 cells replicon)
    Specification
    huh 7 cells replicon
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Type
    Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Jul 30 2008

    Answer

    Thank you for your enquiry. I looked up some Hepatitis C Virus antibodies that may be of interest to you and the information is listed below. It does not appear that we have one that is specific for strain 2a or has even bee tested for reactivity with that strain. Please contact us again if you have any additional questions. Ab20773 - Goat polyclonal to Hepatitis C Virus NS5: Immunogen used was recombinant NS5 a&b genotype 1a (100% of a and 70% of b). This antibody has not been tested for reactivity to genotype 2a. Ab1035 - Rabbit polyclonal to Hepatitis C Virus: Immunogen used was a peptide corresponding to amino acids 33-43 of Hepatitis C Virus (HCV) core protein C3. Ab1033 - Rabbit polyclonal to Hepatitis C Virus: Immunogen used was a peptide corresponding to amino acids 9-21 of Hepatitis C Virus (HCV) core protein C1. For ab1035 and ab1033, strain 2a hasn't been tested, but the understanding is that the sequences derived from core proteins C1 and C3 which were used for immunization are well conserved in the different strains of Hepatitis C virus. Therefore, it is likely that these antibodies will react with Hepatitis C strain 2a. Ab18662 - Mouse monoclonal [24-8 ] to Hepatitis C Virus: Immunogen used was a highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of Chinese HCV strains. This antibody is not hepatitis C virus[HCV] strain specific, and will recognize the NS3 of HCV strains. If the NS3 variation has been observed in this strain, it may cause the weaker/or non reaction when using this antibody, in the case of the variation is matched the epitope region or interfered the Antibody-Antigen binding-reaction site/sequences. Ab27791 - Goat polyclonal to Hepatitis C Virus: Immunogen used was recombinant chimeric HCV polyprotein (60 kDa). This antibody product will react with various HCV, and the reaction is not HCV strain specific.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up