Overview

  • Product name
    Anti-Hepatitis C Virus genotype 1a NS5 antibody
  • Description
    Goat polyclonal to Hepatitis C Virus genotype 1a NS5
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant NS5 a&b genotype 1a (100% of a and 70% of b).

Properties

Applications

Our Abpromise guarantee covers the use of ab20773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. WB: 1/100. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV.
    • Cellular localization
      Endoplasmic reticulum membrane
    • Database links
      • Alternative names
        • HCV 1a NS5 antibody
        • Hepatitis C virus NS5A antibody
        • Non-structural protein 5A antibody
        • p56 antibody

      References

      This product has been referenced in:
      See 1 Publication for this product

      Customer reviews and Q&As

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      Application
      ELISA
      Sample
      Human Cell (huh 7 cells replicon)
      Specification
      huh 7 cells replicon
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
      Type
      Sandwich (Capture)

      Abcam user community

      Verified customer

      Submitted Jul 30 2008

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