Anti-Hepatitis C Virus NS3 antibody (ab21124)
Key features and details
- Goat polyclonal to Hepatitis C Virus NS3
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Hepatitis C virus
- Isotype: IgG
Overview
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Product name
Anti-Hepatitis C Virus NS3 antibody
See all Hepatitis C Virus NS3 primary antibodies -
Description
Goat polyclonal to Hepatitis C Virus NS3 -
Host species
Goat -
Tested applications
Suitable for: ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant full length protein (genotype 1a).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
Sodium sulfate precipitation & ion-exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent dilution.
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ICC/IF |
Use at an assay dependent dilution.
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 72 kDa.
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Notes |
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ELISA
Use at an assay dependent dilution. |
ICC/IF
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. Predicted molecular weight: 72 kDa. |
Target
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Relevance
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa. -
Cellular localization
Endoplasmic reticulum membrane -
Database links
- SwissProt: P26663 Hepatitis C virus
- SwissProt: P26664 Hepatitis C virus
- SwissProt: Q81258 Hepatitis C virus
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Alternative names
- Hepatitis C Virus NS3 antibody
- NS3 antibody
- NS3P antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab21124 has been referenced in 6 publications.
- Chen KY et al. A highly sensitive cell-based luciferase assay for high-throughput automated screening of SARS-CoV-2 nsp5/3CLpro inhibitors. bioRxiv N/A:N/A (2021). PubMed: 34981051
- Wozniak AL et al. Hepatitis C virus promotes virion secretion through cleavage of the Rab7 adaptor protein RILP. Proc Natl Acad Sci U S A 113:12484-12489 (2016). PubMed: 27791088
- Quan H et al. Hepatitis C virus core protein epigenetically silences SFRP1 and enhances HCC aggressiveness by inducing epithelial-mesenchymal transition. Oncogene N/A:N/A (2013). PubMed: 23770846
- Kannan RP et al. Hepatitis C virus infection causes cell cycle arrest at the level of initiation of mitosis. J Virol 85:7989-8001 (2011). WB . PubMed: 21680513
- McGivern DR et al. Impaired replication of hepatitis C virus containing mutations in a conserved NS5B retinoblastoma protein-binding motif. J Virol 83:7422-33 (2009). WB . PubMed: 19458004
- Wang T et al. Role of Hepatitis C virus core protein in viral-induced mitochondrial dysfunction. J Viral Hepat : (2009). WB ; Hepatitis C virus . PubMed: 20002299