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here is the scan of the blot. there is a good band but unfortunately it shows up in the negative control which is the 20.5 and does not show up in the positive control using NS3 protein only. let me know what you think.
Asked on Jan 21 2005
Thank you for sending me the image of your blot. So, you have no bands (just some background) in the NS3 recombinant protein lane, and the same pattern of bands in the other lanes, including the negative control. Just to make sure, do you know that your secondary antibody is working properly? Also, have you tried in the past another Hepatitis C Virus antigen antibody with these samples?
Answered on Jan 25 2005