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Hi, I am interested in the hepatitis C anti-NS3 antibody for WB. I would like to check the expression condition of the NS3 protein using the antibody, especially its helicase domain. Would you suggest any antibody fit for this purpose? I have rabbit/mouse HRP secondary antibody. Thanks. Yi
Asked on Aug 15 2011
Thank you for your enquiry. I am sorry it has taken some time to request the immunogen seuqences from our sources and review these for you, thank you for your patience. I can confirm the following mouse Hepatitis C Virus NS3 antibodies have been tested in wesern blot and should detect the helicase domain. I can recommend to review the online datasheets for further information: ab13830 Hepatitis C Virus NS3 antibody [H23] Mouse monoclonal Applications: ELISA, IHC-P, WB The immunogen for this mab was of the genotype 1b, the exact sequence is unknown. The epitope has been mapped to the helicase domain (between aa positions 1350 and1500). Click here (or use the following: https://www.abcam.com/index.html?datasheet=13830). ab65407 Hepatitis C Virus NS3 antibody [8 G-2] Mouse monoclonal Applications: ELISA, ICC/IF, IHC-P, WB The epitope has been mapped between amino acid positions 1340 and 1480 The antibody includes and recognises the helicase domain. Click here (or use the following: https://www.abcam.com/index.html?datasheet=65407). I am still waiting for confirmation of the immunogen from several more antibodies. I will contact you again if I have any further information that any will detect the helicase domain. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.
Answered on Aug 15 2011