Overview

  • Product name

    Anti-Hepatitis C Virus NS4A antibody [S4-13]
    See all Hepatitis C Virus NS4A primary antibodies
  • Description

    Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Hepatitis C virus
  • Immunogen

    A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • Epitope

    N terminal region of the NS4 protein (NS4A).
  • Positive control

    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab128685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha
  • Cellular localization

    Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease.
  • Database links

    • Alternative names

      • core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • HCV NS4A antibody
      • Non structural protein 4A antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA-dependent RNA polymerase antibody
      • p7 protein antibody
      • polyprotein antibody
      see all

    Images

    • Anti-Hepatitis C Virus NS4A antibody [S4-13] (ab128685) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA
    • ab124528 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a Hepatitis C Virus genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and HCV NS4a protein was detected by indirect immunofluorescence staining using this antibody.

    References

    ab128685 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Huh7 cells)
    Loading amount
    30 µg
    Specification
    Huh7 cells
    Gel Running Conditions
    Reduced Denaturing (15)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted May 16 2012

    Answer

    Thank you for your email. yes, I can send you retroactively the discount code.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab128685 in HCV genotype 2a. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for HCV genotype 2aand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for contacting Abcam and for submitting an Abreview.

    Our testing discount codes are issued before you purchase the antibody, this way we can determine if the application or species that you wish to use is suitable for a testing discount code.

    You can find out more information about how the program works using the link below:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



    If there is anything else I can help you with, please let me know.

    Read More

    Answer

    Thank you for contacting us.

    Here is more information on how our tetsing discount peogram works.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I am considering the genotype 2a as an untested species, and thus, you would qualify for the program.

    Please let me know if I shall send you a testing discount code and for which antibody.

    I look forward to hear back from you.

    Read More

    Answer

    Thank you for your patience.

    I have good news for you: The unconjugated antibody for ab124526 is now in the catalog and available to be ordered. It is ab128685 (Anti-Hepatitis C Virus NS4A antibody [S4-13]):

    https://www.abcam.com/hepatitis-c-virus-ns4a-antibody-s4-13-ab128685.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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