Biotin Anti-Hepatitis C Virus NS4A antibody [S4-13] (ab124526)
Key features and details
- Biotin Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A
- Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-Hepatitis C Virus NS4A antibody [S4-13] -
Description
Biotin Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A -
Host species
Mouse -
Conjugation
Biotin -
Specificity
Specific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.
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Epitope
N terminal region of the NS4 protein (NS4A). -
Positive control
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
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General notes
[biotin] / [IgG] = 11.3
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
S4-13 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha -
Cellular localization
Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease. -
Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P26663 Hepatitis C virus
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Alternative names
- core protein antibody
- E1 protein antibody
- E2 protein antibody
see all
Images
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Biotin Anti-Hepatitis C Virus NS4A antibody [S4-13] (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
Predicted band size: 6 kDa -
ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124526 has not yet been referenced specifically in any publications.