Overview

  • Product name

    Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin)
    See all Hepatitis C Virus NS4A primary antibodies
  • Description

    Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    Specific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes
  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis C virus
  • Immunogen

    A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • Epitope

    N terminal region of the NS4 protein (NS4A).
  • Positive control

    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • General notes

    [biotin] / [IgG] = 11.3

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    S4-13
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha
  • Cellular localization

    Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease.
  • Database links

    • Alternative names

      • core protein antibody
      • E1 protein antibody
      • E2 protein antibody
      • HCV NS4A antibody
      • Non structural protein 4A antibody
      • NS2 protein antibody
      • NS3 protease/helicase antibody
      • NS4A antibody
      • NS4A protein antibody
      • NS4B protein antibody
      • NS5A protein antibody
      • NS5B RNA-dependent RNA polymerase antibody
      • p7 protein antibody
      • polyprotein antibody
      see all

    Images

    • Anti-Hepatitis C Virus NS4A antibody [S4-13] (Biotin) (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.

      Predicted band size: 6 kDa

    • ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.

    References

    ab124526 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for contacting us. In theory the protein ab49029 should react with antibidy ab124526, since the protein is full length Hepatitis C Virus NS4 protein. However, this has not been tested and there is a possibility the tag could prevent the antibody from binding. The ratio of biotin/IgG is 11/4 for the antibody.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for contacting us.

    Here is more information on how our tetsing discount peogram works.

    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I am considering the genotype 2a as an untested species, and thus, you would qualify for the program.

    Please let me know if I shall send you a testing discount code and for which antibody.

    I look forward to hear back from you.

    Read More

    Answer

    Thank you for your patience.

    I have good news for you: The unconjugated antibody for ab124526 is now in the catalog and available to be ordered. It is ab128685 (Anti-Hepatitis C Virus NS4A antibody [S4-13]):

    https://www.abcam.com/hepatitis-c-virus-ns4a-antibody-s4-13-ab128685.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for contacting us.


    I have the following information for you.

    ab13831: This antibody recognizes proteins of genotypes 1a, 1b, 3 and 4, and possibly others, but not 2a. The following antibody recognizes genotype 2a: Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830), but it is not NS4.

    ab124526: Theunconjugated form of the antibody would be available. I am still waiting for more information in order to have it added to our catalog. I will be in touch when I have an update for you- probably next week.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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