Overview

  • Product name
    Anti-Hepatitis C Virus NS5a antibody [H26]
  • Description
    Mouse monoclonal [H26] to Hepatitis C Virus NS5a
  • Host species
    Mouse
  • Specificity
    Hepatitis C Virus antigen NS5a. This antibody works with the HCV genotypes 1a, 1b, 3 and 4, but not with genotype 2a.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Immunogen

    Recombinant full length protein (E. coli).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    H26
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/1000.
ICC/IF 1/50.

Target

  • Relevance
    HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
  • Alternative names
    • HCV antibody
    • HCV Non-structural protein 5A antibody
    • Non-structural protein 5A antibody
    • NS5A antibody
    • p56 antibody
    see all

Images

  • Representative western blot detecting Hepatitis C Virus NS5a with ab13833.

    Amounts of Hepatitis C Virus NS5a were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, delta414-428 or “delta414-428+20aa” mutants.

References

This product has been referenced in:
  • Kuwashiro T  et al. Regulation of interferon signaling and HCV-RNA replication by extracellular matrix. Int J Mol Med 42:957-965 (2018). Read more (PubMed: 29786754) »
  • Madhvi A  et al. A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound. Sci Rep 7:5816 (2017). Read more (PubMed: 28725041) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your message.

Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and freeze them as soon as possible.

We would recommend that products are stored as recommended on the datasheet as soon as they are delivered. This will ensure the product remains stable and that we can continue to provide our guarantee.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry. I can confirm that the exact epitope is not know, but it has been mapped between amino acid positions 2200 and 2250. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up