Key features and details
- Mouse monoclonal [OCH1E5] to Hepatocyte Specific Antigen
- Suitable for: IHC-Fr, IHC-P, ICC/IF
- Reacts with: Dog, Human
- Isotype: IgG1
Product nameAnti-Hepatocyte Specific Antigen antibody [OCH1E5]
See all Hepatocyte Specific Antigen primary antibodies
DescriptionMouse monoclonal [OCH1E5] to Hepatocyte Specific Antigen
SpecificityHepatocyte specific antigens are useful in studying hepatocellular tumors. They may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. They have been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. Hepatcoyte labels an antigen in the mitochondrial fraction of the liver homogenates.
Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IFmore details
Species reactivityReacts with: Dog, Human
Tissue, cells or virus corresponding to Human Hepatocyte Specific Antigen. Extract of a formalin-fixed, rejected-allograft of a Human liver.
- Liver or Hepatocellular Carcinoma.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
Purification notes200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G
Our Abpromise guarantee covers the use of ab190706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
RelevanceHepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
ab190706 has been referenced in 2 publications.
- Bertol BC et al. HLA-G liver expression and HLA-G extended haplotypes are associated with chronic hepatitis C in HIV-negative and HIV-coinfected patients. Clin Immunol 217:108482 (2020). PubMed: 32470543
- Zhang J et al. Overexpression of Epcam and CD133 Correlates with Poor Prognosis in Dual-phenotype Hepatocellular Carcinoma. J Cancer 11:3400-3406 (2020). PubMed: 32231746