Recombinant Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18937] to Hepcidin + Hepcidin-2
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Hepcidin + Hepcidin-2 antibody [EPR18937]
See all Hepcidin + Hepcidin-2 primary antibodies -
Description
Rabbit monoclonal [EPR18937] to Hepcidin + Hepcidin-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse Hepcidin recombinant fragment protein; Mouse Hepcidin-2 recombinant fragment protein; Mouse liver lysate. IHC-P: Mouse liver tissue. IHC-F: Mouse liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18937 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB | (1) |
1/200. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
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IHC-Fr | (1) |
1/100.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/200. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa). |
IHC-Fr
1/100. |
Target
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Relevance
Hepcidin: Q9EQ21: Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. Hepcidin-2: Q80T19: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. -
Database links
- SwissProt: Q80T19 Mouse
- SwissProt: Q9EQ21 Mouse
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Alternative names
- Hamp antibody
- Hamp1 antibody
- Hamp2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on hepatocytes of Mouse liver was observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/200 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 9 kDa
Observed band size: 9 kDa
Exposure time: 20 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Highly expressed in the liver and to a much lesser extent in the heart (PMID:11113132 and PMID:12729891)
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All lanes : Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/5000 dilution
Lane 1 : Mouse Hepcidin recombinant fragment protein
Lane 2 : Mouse Hepcidin-2 recombinant fragment protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 9 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1: 1 second.
Lane 2: 2 seconds.The mouse recombinant fragment proteins contain aa24-83 with a GST-Tag and were made in-house.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized frozen section of Mouse liver labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on hepatocytes of Mouse liver.
Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse kidney. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse spleen. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon cancer. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab190775 has been referenced in 2 publications.
- Wang X et al. An Integrated Systematic Analysis and the Clinical Significance of Hepcidin in Common Malignancies of the Male Genitourinary System. Front Genet 13:771344 (2022). PubMed: 35646093
- Fader KA et al. Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBa/ß activation in aryl hydrocarbon receptor-elicited hepatotoxicity. Toxicol Appl Pharmacol 321:1-17 (2017). PubMed: 28213091