Western blot abreview for Anti-Hepcidin + Hepcidin-2 antibody [EPR18937]

Excellent
Abreviews
Application
Western blot
Sample
Human Serum (Human serum and LPS treated rat serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
rat treated with 5mg/kg LPS and then serum collected aftrer 4 hours
Specification
Human serum and LPS treated rat serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1M TBST with 1%milk
Dilution
1/200

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: DAKO anti rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
Embryionic brain lysate
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 2.8 kDa and 10kDa kDa
Positive control
Human serum and LPS treated rat serum

Additional data

Additional Notes
Good antibody, recognised both hepcidin and pro-hepcidin band in human and rat serum. Lane 1: Marker, Lane 2: Hepcidin peptide, Lane 3: control serum, Lane 4: LPS-treated blood, Lane 5: Embryonic body lysate.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Dec 08 2015

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