Key features and details
- Rabbit polyclonal to Hepcidin-25
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Hepcidin-25 antibody
See all Hepcidin-25 primary antibodies
DescriptionRabbit polyclonal to Hepcidin-25
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- WB: Recombinant Hepcidin protein. IHC-P: Human liver tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab30760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionLiver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
Tissue specificityHighest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
Involvement in diseaseHemochromatosis 2B
Sequence similaritiesBelongs to the hepcidin family.
- Information by UniProt
FormHepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 3 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.
Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
ab30760 has been referenced in 20 publications.
- Zuo E et al. Increased expression of hepcidin and associated upregulation of JAK/STAT3 signaling in human gastric cancer. Oncol Lett 15:2236-2244 (2018). IHC-P . PubMed: 29434930
- van Swelm RPL et al. Endogenous hepcidin synthesis protects the distal nephron against hemin and hemoglobin mediated necroptosis. Cell Death Dis 9:550 (2018). PubMed: 29749404
- Jang M et al. Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform. Biomed Microdevices 20:54 (2018). PubMed: 29946898
- Blanchette-Farra N et al. Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer. Oncogene 37:4013-4032 (2018). PubMed: 29695834
- Liu J et al. Studying hepcidin and related pathways in osteoblasts using a mouse model with insulin receptor substrate 1-loss of function. Mol Med Rep 17:350-357 (2018). WB, IHC-P ; Mouse . PubMed: 29115497