ELISA abreview for Anti-Hepcidin-25 antibody

Good
Abreviews
Application
ELISA
Sample
Mouse Recombinant protein (Mouse cell lysate)
Specification
Mouse cell lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Type
Indirect

Other product details

Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS/1% Tween-20

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/25000

Additional data

Additional Notes
Cell Lysates prepared in carbonate coating buffer ([2.93g Sodium Bicarbonate (NaHCO3), 1.59g Sodium carbonate (Na2CO3)] in 1 litre deionised water, pH 9.6) for 1 hour at 37 degress.

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Submitted Oct 15 2012

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