The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa).
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005). Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
Hepcidin 20 antibody
Hepcidin 25 antibody
Hepcidin antimicrobial peptide antibody
LEAP 1 antibody
Liver expressed antimicrobial peptide antibody
Liver-expressed antimicrobial peptide 1 antibody
Putative liver tumor regressor antibody
Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)
Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/5000 dilution + Human Hepcidin fragment protein at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution