Product nameAnti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free
See all Hepcidin-25 primary antibodies
DescriptionRabbit monoclonal [EPR18074] to Hepcidin-25 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Hepcidin-25 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P81172
- WB: Human plasma, serum and liver lysate.
Ab238974 is the carrier-free version of ab187778. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 9, 3 kDa (predicted molecular weight: 9 kDa).|
FunctionLiver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
Tissue specificityHighest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
Involvement in diseaseHemochromatosis 2B
Sequence similaritiesBelongs to the hepcidin family.
- Information by UniProt
FormHepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
All lanes : Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human serum
Lane 3 : Human liver lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Predicted band size: 9 kDa
Observed band size: 3 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187778).
ab238974 has not yet been referenced specifically in any publications.