• Product name
    HepG2 nuclear extract lysate
    See all HepG2 lysates
  • General notes
    Cell line: HepG2 (Human hepatocellular carcinoma).
    We recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applications
    Suitable for: EMSA, WBmore details


  • Form
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 1mM Magnesium Chloride, 100mM KCl, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    Hepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).


Our Abpromise guarantee covers the use of ab14660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.


ab14660 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Q&A


Thank you for your technical support and interest in our products.

As the datasheet indicates, the recommended positive control for ab89220: Anti-Tbx3 antibody is HepG2 cell lysate for Western blot application.

I have conducted a search for you and found 8 HepG2 cell lysate in our catalogue. You can use whole cell lyaste such as ab7900 or nuclear lysate (untreated) such as ab14660. Since Tbx3 is localised in the nuclear, it may be worth using the nucler fraction and enriched lysate.

I would advise you to take a look at these products to see which one would suit your need best:

https://www.abcam.com/ab7900 HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

https://www.abcam.com/ab14660: HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

If you need any further assistance in the future, please do not hesitate to contact me.

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Thank you for your enquiry. The nuclear extracts were prepared as detailed in: Dignam, J.D., Lebovitz, R.M. and Roeder, R.G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucl. Acids Res. 11, 1475–89. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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