Overview

  • Product name

  • Description

    Rabbit polyclonal to Hepsin/HPN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Hepsin/HPN aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125234)

  • Positive control

    • Recombinant Human Hepsin/HPN protein (ab114907) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 whole cell lysate.
  • General notes

     This product was previously labelled as Hepsin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab73133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Plays an essential role in cell growth and maintenance of cell morphology.
  • Tissue specificity

    Present in most tissues, with the highest level in liver.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.4.21. antibody
    • HEPS antibody
    • HEPS_HUMAN antibody
    • Hepsin antibody
    • Hpn antibody
    • Serine protease hepsin antibody
    • Serine protease hepsin catalytic chain antibody
    • Serine protease hepsin non catalytic chain antibody
    • TMPRSS 1 antibody
    • TMPRSS1 antibody
    • Transmembrane protease serine 1 antibody
    see all

Images

  • Anti-Hepsin/HPN antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    Hepsin/HPN contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.

References

ab73133 has not yet been referenced specifically in any publications.

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