Product nameAnti-Hepsin/HPN antibody
See all Hepsin/HPN primary antibodies
DescriptionRabbit polyclonal to Hepsin/HPN
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Macaque monkey, Orangutan
Synthetic peptide corresponding to Human Hepsin/HPN aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Hepsin/HPN protein (ab114907) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 whole cell lysate.
This product was previously labelled as Hepsin
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionPlays an essential role in cell growth and maintenance of cell morphology.
Tissue specificityPresent in most tissues, with the highest level in liver.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
- Information by UniProt
- EC 3.4.21. antibody
- HEPS antibody
- HEPS_HUMAN antibody
Anti-Hepsin/HPN antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
Hepsin/HPN contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.
ab73133 has not yet been referenced specifically in any publications.