Recombinant Anti-Hepsin/HPN antibody [EP7654] (ab189246)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7654] to Hepsin/HPN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hepsin/HPN antibody [EP7654]
See all Hepsin/HPN primary antibodies -
Description
Rabbit monoclonal [EP7654] to Hepsin/HPN -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat liver and kidney tissue lysates. IHC-P: Human liver and stomach tissues.
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General notes
This product has switched from a hybridoma to recombinant production method on 24th October 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7654 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Notes |
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WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Plays an essential role in cell growth and maintenance of cell morphology. -
Tissue specificity
Present in most tissues, with the highest level in liver. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3249 Human
- Entrez Gene: 15451 Mouse
- Entrez Gene: 29135 Rat
- Omim: 142440 Human
- SwissProt: P05981 Human
- SwissProt: O35453 Mouse
- SwissProt: Q05511 Rat
- Unigene: 182385 Human
see all -
Alternative names
- EC 3.4.21. antibody
- HEPS antibody
- HEPS_HUMAN antibody
see all
Images
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All lanes : Anti-Hepsin/HPN antibody [EP7654] (ab189246) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human kidney lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1 and 2: 20 seconds; Lanes 3 and 4: 120 seconds; Lanes 5 and 6: 80 seconds
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Immunohistochemistry analysis of paraffin-embedded Human liver tissue sections labelling Hepsin/HPN with ab189246 at 1/1000 dilution. The section was incubated with ab189246 at 4°C overnight. Ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Cytoplasmic staining on human liver tissue.
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Immunohistochemistry analysis of paraffin-embedded Human stomach tissue sections labelling Hepsin/HPN with ab189246 at 1/1000 dilution. The section was incubated with ab189246 at 4°C overnight. Ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Cytoplasmic staining on human stomach tissue.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab189246 has been referenced in 3 publications.
- Sun S et al. N-glycan in the scavenger receptor cysteine-rich domain of hepsin promotes intracellular trafficking and cell surface expression. Int J Biol Macromol 161:818-827 (2020). PubMed: 32553951
- Wang X et al. LncRNA TRPC7-AS1 regulates nucleus pulposus cellular senescence and ECM synthesis via competing with HPN for miR-4769-5p binding. Mech Ageing Dev 190:111293 (2020). PubMed: 32585234
- Li R et al. Hepsin Promotes Epithelial-Mesenchymal Transition and Cell Invasion Through the miR-222/PPP2R2A/AKT Axis in Prostate Cancer. Onco Targets Ther 13:12141-12149 (2020). PubMed: 33268993