Anti-HER2 ELISA Kit (ab237645)
Overview
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Product name
Anti-HER2 ELISA Kit
See all Trastuzumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 15% Inter-assay Sample n Mean SD CV% Overall < 15% -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
11 ng/ml -
Range
11 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Trastuzumab ELISA Kit (Herceptin®) (ab237645) is a highly specific and sensitive kit designed for the in vitro determination of Trastuzumab in biological matrices such as human serum and plasma. The density of color is proportional to the amount of Trastuzumab captured from the samples and can be quantified when compared with standard curve.
Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has anti-tumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 1 x 25ml HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Trastuzumab Standard S1 1 x 300µl Trastuzumab Standard S2 1 x 300µl Trastuzumab Standard S3 1 x 300µl Trastuzumab Standard S4 1 x 300µl Trastuzumab Standard S5 1 x 300µl Trastuzumab Standard S6 1 x 300µl Trastuzumab Standard S7 1 x 300µl Wash buffer (20X) 1 x 50ml -
Relevance
Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has antitumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes. B -
Alternative names
- Herceptin
- Herclon
- human epidermal growth factor receptor 2
Images
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Example of Trastuzumab standard curveTypical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.
References
ab237645 has not yet been referenced specifically in any publications.
