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    her2-elisa-kit-ab237645.pdf

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Anti-HER2 ELISA Kit  (ab237645)

  • Datasheet
  • SDS
  • Protocol Booklet
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Example of standard curve

    Key features and details

    • Sensitivity: 11 ng/ml
    • Range: 11 ng/ml - 300 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Quantitative
    • Reacts with: Human

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    Overview

    • Product name

      Anti-HER2 ELISA Kit 
      See all Anti-HER2 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 15%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 15%
    • Sample type

      Serum, Plasma
    • Assay type

      Quantitative
    • Sensitivity

      11 ng/ml
    • Range

      11 ng/ml - 300 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 85% - 115%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      anti-HER2 ELISA Kit (ab237645) is designed to quantify/measure the antibody against HER2 with high specificity and sensitivity in biological matrices.
    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called E4376 BioSim™ HER2 mab (Human) ELISA Kit. E4376-100 is the same size as the 96 test size of ab237645.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 96 tests
      Anti-HER2 Standard S1 1 x 300µl
      Anti-HER2 Standard S2 1 x 300µl
      Anti-HER2 Standard S3 1 x 300µl
      Anti-HER2 Standard S4 1 x 300µl
      Anti-HER2 Standard S5 1 x 300µl
      Anti-HER2 Standard S6 1 x 300µl
      Anti-HER2 Standard S7 1 x 300µl
      Assay Buffer 2 x 50ml
      HRP-conjugate Probe 1 x 12ml
      Micro ELISA Plate 1 unit
      Plate sealers 2 units
      Stop Solution 1 x 12ml
      TMB substrate 1 x 12ml
      Wash buffer (20X) 1 x 50ml
    • Relevance

      This product is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). It has anti-tumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. This antibody was approved in 1998 for clinical use for HER2 overexpressing metastatic breast cancer. In HER2 overexpressing cells, it markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes.
    • Alternative names

      • human epidermal growth factor receptor 2

    Images

    • Example of standard curve
      Example of standard curve

      Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab237645? Please let us know so that we can cite the reference in this datasheet.

    ab237645 has not yet been referenced specifically in any publications.

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