• Product name
  • Description
    Rabbit polyclonal to HERC2
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 4784-4834 of Human HERC2 (NP_004658.3)

  • Positive control
    • Whole cell lysate from HeLa cells.



Our Abpromise guarantee covers the use of ab85832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 527 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function
    E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Mental retardation, autosomal recessive 38
  • Sequence similarities
    Contains 1 cytochrome b5 heme-binding domain.
    Contains 1 DOC domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 MIB/HERC2 domain.
    Contains 21 RCC1 repeats.
    Contains 1 ZZ-type zinc finger.
  • Domain
    The ZZ-type zinc finger mediates binding to SUMO1, and at lowe level SUMO2.
    The RCC1 repeats are grouped into three seven-bladed beta-propeller regions.
  • Post-translational
    Phosphorylation at Thr-4827 is required for interaction with RNF8.
    Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus. Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • D15F37S1 antibody
    • E3 ubiquitin-protein ligase HERC2 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase 2 antibody
    • HECT domain and RCC1 like domain containing protein 2 antibody
    • HECT domain and RCC1-like domain-containing protein 2 antibody
    • Hect domain and RLD 2 antibody
    • HERC2 antibody
    • HERC2_HUMAN antibody
    • jdf2 antibody
    • MRT38 antibody
    • p528 antibody
    • Probable E3 ubiquitin-protein ligase HERC2 antibody
    • SHEP1 antibody
    see all


  • All lanes : Anti-HERC2 antibody (ab85832) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique.

    Predicted band size: 527 kDa
    Observed band size: 527 kDa
    Additional bands at: 160 kDa, 260 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes
  • Immunoprecipitation/ Western Blot
    For IP:
    Lane 1: ab85832 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab85832 at 1µg/ml for WB
    Whole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.


This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Le laboratoire vient de m'informer que le gel utilisé avec l'anti-HERC2 ab85832 en western blot est : 3-8% Nupage Tris-Acetate Mini gels, la membrane utilisée est de type nitrocellulose. J'espère que ces informations, ainsi que le ...

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Veuillez trouver ci-dessous le protocole (en anglais)que nous recommandons pour le transfert de protéines de haut poids moléculaire en western blot : -We wouldsuggest to optimize the transfer conditions by increasing the transfer time to ...

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