Key features and details
- Rabbit polyclonal to HERC4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HERC4 antibody
See all HERC4 primary antibodies
DescriptionRabbit polyclonal to HERC4
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: RT4 and U-251 cell lysates. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 119 kDa.|
FunctionProbable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Tissue specificityExpressed in brain and testis and detected in heart and placenta.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 7 RCC1 repeats.
Developmental stageExpressed in fetal brain.
Cellular localizationCytoplasm > cytosol. shows a punctate cytoplasmic distribution.
- Information by UniProt
- DKFZP564G092 antibody
- HECT and RLD domain containing E3 ubiquitin protein ligase 4 antibody
- HECT domain and RCC1 like domain containing protein 4 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for HERC4 (green) using ab221757 at 4 µg/ml in ICC/IF.
All lanes : Anti-HERC4 antibody (ab221757) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 119 kDa
ab221757 has not yet been referenced specifically in any publications.