• Product name

  • Description

    Rabbit polyclonal to HERC4
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1007 and 1057 of Human HERC4 (NP_071362.1).

  • Positive control

    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.



Our Abpromise guarantee covers the use of ab85732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 119 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
  • Tissue specificity

    Expressed in brain and testis and detected in heart and placenta.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 7 RCC1 repeats.
  • Developmental stage

    Expressed in fetal brain.
  • Cellular localization

    Cytoplasm > cytosol. shows a punctate cytoplasmic distribution.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZP564G092 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase 4 antibody
    • HECT domain and RCC1 like domain containing protein 4 antibody
    • HECT domain and RCC1-like domain-containing protein 4 antibody
    • Hect domain and RLD 4 antibody
    • HERC 4 antibody
    • HERC4 antibody
    • HERC4_HUMAN antibody
    • KIAA1593 antibody
    • Probable E3 ubiquitin protein ligase HERC4 antibody
    • Probable E3 ubiquitin-protein ligase HERC4 antibody
    see all


  • All lanes : Anti-HERC4 antibody (ab85732) at 0.1 µg/ml

    Lane 1 : Whole cell
    lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell
    lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell
    lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell
    lysate from 293T cells at 50 µg
    Lane 5 : Whole cell
    lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 119 kDa
    Observed band size: 119 kDa

    Exposure time: 30 seconds
  • Detection of Human HERC4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85732 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.


ab85732 has not yet been referenced specifically in any publications.

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