Overview

  • Product name
    Anti-HERC6 antibody
  • Description
    Rabbit polyclonal to HERC6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 123 - 393 of Human HERC6 (BC035775).

  • Positive control
    • WB: HeLa cell lysate. IHC-P: fetal testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab99949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
  • Tissue specificity
    Detected in brain, heart, placenta and testis.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 5 RCC1 repeats.
  • Developmental stage
    Expressed in fetal brain.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • EC 6.3.2. antibody
    • FLJ20637 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 antibody
    • HECT domain and RCC1 like domain containing protein 6 antibody
    • HECT domain and RCC1-like domain 6 antibody
    • HECT domain and RCC1-like domain-containing protein 6 antibody
    • Hect domain and RLD 6 antibody
    • HERC 6 antibody
    • HERC6 antibody
    • HERC6_HUMAN antibody
    • Potential ubiquitin ligase antibody
    • Probable E3 ubiquitin protein ligase HERC6 antibody
    • Probable E3 ubiquitin-protein ligase HERC6 antibody
    see all

Images

  • Western blot - Anti-HERC6 antibody (ab99949)
    Western blot - Anti-HERC6 antibody (ab99949)
    Anti-HERC6 antibody (ab99949) at 1/1000 dilution + HeLa cell lysate

    Predicted band size: 115 kDa
    Observed band size: 115 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (ab99949)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (ab99949)
    ab99949 at 1/100 dilution detecting HERC6 expression in formalin-fixed, paraffin-embedded Human fetal testis tissue.
    Note cytoplasmic staining.

References

ab99949 has not yet been referenced specifically in any publications.

Publishing research using ab99949? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Thanks for your prompt reply and help. If possible, I would like to have
the purchase amount refunded, by crediting it to our institutional credit
card (which was used for the purchase).

Please let me know if you need anything else.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact o...

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Inquiry: Sample: 40 ug of total protein from control or HERC6 shRNA knockdown HeLa cells (RIPA lysates) were loaded on 7% SDS-PAGE blocking: 5% non-fat milk detection: Femto reagent (Pierce) primary antibody dilution 1:1000 in TBST+ 0.3% BSA + 0.02% Na...

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Thank you for your enquiry.
I am sorry to hear this vial of ab99949 is giving you trouble. I am curious to know if you have successfully blotted the HeLa cell lysate with any other antibodies.
Thanks in advance for the additional information!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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