Anti-HERC6 antibody (ab99949)

Reviews (1)Q&A (2)
Western blot - Anti-HERC6 antibody (ab99949)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (ab99949)

Key features and details

  • Rabbit polyclonal to HERC6
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HERC6 antibody

  • Description

    Rabbit polyclonal to HERC6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment, corresponding to amino acids 123 - 393 of Human HERC6 (BC035775).

  • Positive control

    • WB: HeLa cell lysate. IHC-P: fetal testis tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-P
1/100 - 1/500.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-P
1/100 - 1/500.

Target

  • Function

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

  • Tissue specificity

    Detected in brain, heart, placenta and testis.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 5 RCC1 repeats.

  • Developmental stage

    Expressed in fetal brain.

  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 6.3.2. antibody
    • FLJ20637 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase family member 6 antibody
    • HECT domain and RCC1 like domain containing protein 6 antibody
    • HECT domain and RCC1-like domain 6 antibody
    • HECT domain and RCC1-like domain-containing protein 6 antibody
    • Hect domain and RLD 6 antibody
    • HERC 6 antibody
    • HERC6 antibody
    • HERC6_HUMAN antibody
    • Potential ubiquitin ligase antibody
    • Probable E3 ubiquitin protein ligase HERC6 antibody
    • Probable E3 ubiquitin-protein ligase HERC6 antibody
    see all

Images

  • Western blot - Anti-HERC6 antibody (ab99949)
    Western blot - Anti-HERC6 antibody (ab99949)
    Anti-HERC6 antibody (ab99949) at 1/1000 dilution + HeLa cell lysate

    Predicted band size: 115 kDa
    Observed band size: 115 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (ab99949)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (ab99949)
    ab99949 at 1/100 dilution detecting HERC6 expression in formalin-fixed, paraffin-embedded Human fetal testis tissue.
    Note cytoplasmic staining.

References (0)

Publishing research using ab99949? Please let us know so that we can cite the reference in this datasheet.

ab99949 has not yet been referenced specifically in any publications.

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1-3 of 3 Abreviews or Q&A

Imaging Mass Cytometry™ abreview for Anti-HERC6 antibody

 Good
Abreviews
abreview image
Application
Mass Cytometry
Sample
Human Tissue sections (Liver)
Specification
Liver
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Abcam user community

Verified customer

Submitted Aug 19 2020

Question

Thanks for your prompt reply and help. If possible, I would like to have
the purchase amount refunded, by crediting it to our institutional credit
card (which was used for the purchase).

Please let me know if you need anything else.

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Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question

Inquiry: Sample: 40 ug of total protein from control or HERC6 shRNA knockdown HeLa cells (RIPA lysates) were loaded on 7% SDS-PAGE blocking: 5% non-fat milk detection: Femto reagent (Pierce) primary antibody dilution 1:1000 in TBST+ 0.3% BSA + 0.02% NaN3, over night at 4 degree secondary antibody 1:1000 in TBST, 1h at RT wash steps always in-between 3x 10 minutes at RT in TBST Results: antibody detected over-expressed HERC6 in HEK293T cells without any problem, but there was no specific endogenous HERC6 band in HeLa

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Answer

Thank you for your enquiry.
I am sorry to hear this vial of ab99949 is giving you trouble. I am curious to know if you have successfully blotted the HeLa cell lysate with any other antibodies.
Thanks in advance for the additional information!

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