Recombinant Anti-HERPUD1 antibody [EPR9649] (ab150424)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9649] to HERPUD1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HERPUD1 antibody [EPR9649]
See all HERPUD1 primary antibodies -
Description
Rabbit monoclonal [EPR9649] to HERPUD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human HERPUD1 aa 300-400 (internal sequence). The exact sequence is proprietary.
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Positive control
- BxPC-3, HepG2, SH-SY5Y and 293T cell lysates; BxPC-3 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9649 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid protein 40 generation. -
Tissue specificity
Widely expressed; in the brain, expression seems to be restricted to neurons and vascular smooth muscle cells. Present in activated microglia in senile plaques in the brain of patients with Alzheimer disease. -
Sequence similarities
Contains 1 ubiquitin-like domain. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 9709 Human
- Entrez Gene: 64209 Mouse
- Entrez Gene: 85430 Rat
- Omim: 608070 Human
- SwissProt: Q15011 Human
- SwissProt: Q9JJK5 Mouse
- Unigene: 146393 Human
- Unigene: 29151 Mouse
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Alternative names
- HERP antibody
- HERP1_HUMAN antibody
- HERPUD1 antibody
see all
Images
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All lanes : Anti-HERPUD1 antibody [EPR9649] (ab150424) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
Immunofluorescent analysis of BxPC-3 cells labelling HERPUD1 with ab150424 at 1/100 dilution.
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Intracellular flow cytometric analysis of permeabilized HepG2 cells labelling HERPUD1 with ab150424 at 1/10 dilution (red) or a rabbit IgG (negative).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab150424 has been referenced in 8 publications.
- Vargas G et al. Negative Modulation of Macroautophagy by Stabilized HERPUD1 is Counteracted by an Increased ER-Lysosomal Network With Impact in Drug-Induced Stress Cell Survival. Front Cell Dev Biol 10:743287 (2022). PubMed: 35309917
- Li W et al. The P300/XBP1s/Herpud1 axis promotes macrophage M2 polarization and the development of choroidal neovascularization. J Cell Mol Med 25:6709-6720 (2021). PubMed: 34057287
- Echavarría-Consuegra L et al. Manipulation of the unfolded protein response: A pharmacological strategy against coronavirus infection. PLoS Pathog 17:e1009644 (2021). PubMed: 34138976
- Fenech EJ et al. Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling. Elife 9:N/A (2020). PubMed: 32614325
- Garner KL et al. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress. Sci Rep 8:3902 (2018). PubMed: 29500363
- Lin H et al. Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching. Int J Cardiol 269:242-249 (2018). PubMed: 30017525
- Schulz J et al. Conserved cytoplasmic domains promote Hrd1 ubiquitin ligase complex formation for ER-associated degradation (ERAD). J Cell Sci 130:3322-3335 (2017). PubMed: 28827405
- Bai YX et al. Extrinsic Calcitonin Gene-Related Peptide Inhibits Hyperoxia-Induced Alveolar Epithelial Type II Cells Apoptosis, Oxidative Stress, and Reactive Oxygen Species (ROS) Production by Enhancing Notch 1 and Homocysteine-Induced Endoplasmic Reticulum Protein (HERP) Expression. Med Sci Monit 23:5774-5782 (2017). PubMed: 29206808