Product nameAnti-HERPUD1 antibody [EPR9649]
See all HERPUD1 primary antibodies
DescriptionRabbit monoclonal [EPR9649] to HERPUD1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HERPUD1 aa 300-400 (internal sequence). The exact sequence is proprietary.
- BxPC-3, HepG2, SH-SY5Y and 293T cell lysates; BxPC-3 and HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 44 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionComponent of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid protein 40 generation.
Tissue specificityWidely expressed; in the brain, expression seems to be restricted to neurons and vascular smooth muscle cells. Present in activated microglia in senile plaques in the brain of patients with Alzheimer disease.
Sequence similaritiesContains 1 ubiquitin-like domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- HERP antibody
- HERP1_HUMAN antibody
- HERPUD1 antibody
All lanes : Anti-HERPUD1 antibody [EPR9649] (ab150424) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa
Immunofluorescent analysis of BxPC-3 cells labelling HERPUD1 with ab150424 at 1/100 dilution.
Flow cytometric analysis of permeabilized HepG2 cells labelling HERPUD1 with ab150424 at 1/10 dilution (red) or a rabbit IgG (negative).
This product has been referenced in:
- Garner KL et al. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress. Sci Rep 8:3902 (2018). Read more (PubMed: 29500363) »
- Lin H et al. Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching. Int J Cardiol 269:242-249 (2018). Read more (PubMed: 30017525) »