Overview

  • Product name

    Anti-HERPUD1 antibody [EPR9649]
    See all HERPUD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9649] to HERPUD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HERPUD1 aa 300-400 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • BxPC-3, HepG2, SH-SY5Y and 293T cell lysates; BxPC-3 and HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid protein 40 generation.
    • Tissue specificity

      Widely expressed; in the brain, expression seems to be restricted to neurons and vascular smooth muscle cells. Present in activated microglia in senile plaques in the brain of patients with Alzheimer disease.
    • Sequence similarities

      Contains 1 ubiquitin-like domain.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • HERP antibody
      • HERP1_HUMAN antibody
      • HERPUD1 antibody
      • Homocysteine inducible endoplasmic reticulum stress inducible ubiquitin like domain member 1 antibody
      • Homocysteine inducible endoplasmic reticulum stress inducible ubiquitin like domain member 1 protein antibody
      • Homocysteine responsive endoplasmic reticulum resident ubiquitin like domain member 1 antibody
      • Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein antibody
      • KIAA0025 antibody
      • Methyl methanesulfonate (MMF) inducible fragment protein 1 antibody
      • Methyl methanesulfonate (MMF)-inducible fragment protein 1 antibody
      • MIF1 antibody
      • MMS inducible antibody
      • SUP antibody
      see all

    Images

    • All lanes : Anti-HERPUD1 antibody [EPR9649] (ab150424) at 1/1000 dilution

      Lane 1 : BxPC-3 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • Immunofluorescent analysis of BxPC-3 cells labelling HERPUD1 with ab150424 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized HepG2 cells labelling HERPUD1 with ab150424 at 1/10 dilution (red) or a rabbit IgG (negative).

    References

    This product has been referenced in:

    • Garner KL  et al. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress. Sci Rep 8:3902 (2018). Read more (PubMed: 29500363) »
    • Lin H  et al. Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching. Int J Cardiol 269:242-249 (2018). Read more (PubMed: 30017525) »
    See all 4 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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