Anti-HERV-FRD antibody (ab230235)
Key features and details
- Rabbit polyclonal to HERV-FRD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HERV-FRD antibody
See all HERV-FRD primary antibodies -
Description
Rabbit polyclonal to HERV-FRD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan -
Immunogen
Recombinant fragment corresponding to Human HERV-FRD aa 1-250.
Database link: P60508 -
Positive control
- WB: HeLa, HepG2 and A431 whole cell lysate. IHC-P: Human kidney and adrenal gland tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
|
|
IHC-P |
1/20 - 1/200.
|
Notes |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
IHC-P
1/20 - 1/200. |
Target
-
Function
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties. Can make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity.
SU mediates receptor recognition.
TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane. -
Tissue specificity
Expressed at higher level in placenta. Expressed at lower level in adrenal, bone marrow, brain, breast, colon, kidney, lung, ovary, peripheral blood lymphocytes, prostate, skin, spleen, testis, thymus, thyroid, trachea. -
Sequence similarities
Belongs to the gamma type-C retroviral envelope protein family. HERV class-I FRD env subfamily. -
Domain
Contains the CKS-17 immunosuppressive domain present in many retroviral envelope proteins. As a synthetic peptide, it inhibits immune function in vitro and in vivo. -
Post-translational
modificationsSpecific enzymatic cleavages in vivo yield the mature SU and TM proteins.
The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion. -
Cellular localization
Cell membrane and Virion. - Information by UniProt
-
Database links
- Entrez Gene: 2086 Human
- Entrez Gene: 30816 Human
- Entrez Gene: 405754 Human
- Omim: 610524 Human
- SwissProt: O42043 Human
- SwissProt: O71037 Human
- SwissProt: P10267 Human
- SwissProt: P60507 Human
see all -
Alternative names
- EFRD1_HUMAN antibody
- Endogenous retrovirus group FRD member 1 antibody
- Envelope polyprotein antibody
see all
Images
-
All lanes : Anti-HERV-FRD antibody (ab230235) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Paraffin-embedded human kidney tissue stained for HERV-FRD with ab230235 at 1/100 dilution in immunohistochemical analysis.
-
Paraffin-embedded human adrenal gland tissue stained for HERV-FRD with ab230235 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab230235 has been referenced in 1 publication.
- Li H et al. Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing. Cells 12:N/A (2022). PubMed: 36611882