Product nameAnti-HERV-FRD antibody
See all HERV-FRD primary antibodies
DescriptionRabbit polyclonal to HERV-FRD
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Gorilla, Orangutan
Recombinant fragment corresponding to Human HERV-FRD aa 16-250.
AYRHPDFPLLEKAQQLLQSTGSPYSTNCWLCTSSSTETPGTAYPASPREW TSIEAELHISYRWDPNLKGLMRPANSLLSTVKQDFPDIRQKPPIFGPIFT NINLMGIAPICVMAKRKNGTNVGTLPSTVCNVTFTVDSNQQTYQTYTHNQ FRHQPRFPKPPNITFPQGTLLDKSSRFCQGRPSSCSTRNFWFRPADYNQC LQISNLSSTAEWVLLDQTRNSLFWENKTKGANQSQ
Database link: P60508
- WB: HeLa, HepG2 and A431 whole cell lysate. IHC-P: Human kidney and adrenal gland tissue.
Previously labeled as HERV.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionRetroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties. Can make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity.
SU mediates receptor recognition.
TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
Tissue specificityExpressed at higher level in placenta. Expressed at lower level in adrenal, bone marrow, brain, breast, colon, kidney, lung, ovary, peripheral blood lymphocytes, prostate, skin, spleen, testis, thymus, thyroid, trachea.
Sequence similaritiesBelongs to the gamma type-C retroviral envelope protein family. HERV class-I FRD env subfamily.
DomainContains the CKS-17 immunosuppressive domain present in many retroviral envelope proteins. As a synthetic peptide, it inhibits immune function in vitro and in vivo.
modificationsSpecific enzymatic cleavages in vivo yield the mature SU and TM proteins.
The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion.
Cellular localizationCell membrane and Virion.
- Information by UniProt
- EFRD1_HUMAN antibody
- Endogenous retrovirus group FRD member 1 antibody
- Envelope polyprotein antibody
All lanes : Anti-HERV-FRD antibody (ab230235) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Paraffin-embedded human kidney tissue stained for HERV-FRD with ab230235 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for HERV-FRD with ab230235 at 1/100 dilution in immunohistochemical analysis.
ab230235 has not yet been referenced specifically in any publications.