Product nameAnti-Hes1 antibody [7H11]
See all Hes1 primary antibodies
DescriptionMouse monoclonal [7H11] to Hes1
Tested applicationsSuitable for: ChIP, Flow Cyt, WB, IP, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
His-tagged recombinant human Hes1 protein purified from E.coli
- BeWo cell lysate, HT-29 cell lysate
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR291944 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, HEPES
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab70576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.|
|IP||Use at an assay dependent concentration.
|ELISA||Use at an assay dependent concentration.|
FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
- Information by UniProt
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
All lanes : Anti-Hes1 antibody [7H11] (ab70576) at 1/500 dilution
Lane 1 : BeWo cell lysate
Lane 2 : HT-29 cell lysate
Predicted band size: 30 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Overlay histogram showing SH-SY5Y cells stained with ab70576 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Zhu L et al. Dai-Zong-Fang, A Traditional Chinese Herbal Formula, Ameliorates Insulin Resistance in db/db Mice. Front Physiol 9:224 (2018). Read more (PubMed: 29593575) »
- Moutal A et al. CRMP5 Controls Glioblastoma Cell Proliferation and Survival through Notch-Dependent Signaling. Cancer Res 75:3519-28 (2015). Read more (PubMed: 26122847) »