Overview

  • Product name
  • Description
    Rabbit polyclonal to Hes1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI, corresponding to internal sequence amino acids 1-50 of Human Hes1

  • Positive control
    • Raji cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
    The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb39 antibody
    • C-HAIRY1 antibody
    • c-hairy1A antibody
    • Class B basic helix-loop-helix protein 39 antibody
    • FLJ20408 antibody
    • Hairy and enhancer of split 1 (Drosophila) antibody
    • Hairy and enhancer of split 1 antibody
    • Hairy homolog (Drosophila) antibody
    • Hairy homolog antibody
    • Hairy like antibody
    • Hairy, Drosophila, homolog of antibody
    • Hairy-like protein antibody
    • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
    • HAIRY1 antibody
    • HES-1 antibody
    • hes1 antibody
    • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
    • HES1_HUMAN antibody
    • HHL antibody
    • HL antibody
    • HRY antibody
    • MGC129109 antibody
    • OTTHUMP00000209031 antibody
    • RHL antibody
    • Transcription factor HES-1 antibody
    see all

Images

  • ab49170 (4µg/ml) staining Hes1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining in plates of hepatic cells. However there is stronger nuclear staining in the plates of hepatic cells and hepatic arteries.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab49170 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49170, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Hes1 antibody (ab49170) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa
    Additional bands at: 17 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Western Blot using lysate prepared from Hes1 transfected Ls174 cells. ab49170 used at a 1µg/ml.
  • Western blot using lysate prepared from murine embryonic cells.
    ab49170 used at a 1/800 dilution and the secondary was used at a 1/5000 dilution.
  • ab49170 at 50µg/ml staining Hes1 in Ls174T cells by Immunocytochemistry/ Immunofluorescence.

References

This product has been referenced in:
  • Zhao Z  et al. SPHK1 promotes metastasis of thyroid carcinoma through activation of the S1P/S1PR3/Notch signaling pathway. Exp Ther Med 15:5007-5016 (2018). Read more (PubMed: 29805524) »
  • Wang YC  et al. Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study. Can J Gastroenterol Hepatol 2018:4149317 (2018). Read more (PubMed: 29785394) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
mouse pancreas
Blocking step
casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (E6.5 embryo cranial section)
Permeabilization
Yes - Citrate buffer antigen retrieval
Specification
E6.5 embryo cranial section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Carnoy's fixative

Abcam user community

Verified customer

Submitted Nov 24 2015

Answer

Thank you for contacting us.

You are correct in saying that this product is not covered by our Abpromise guarantee because we have not tested it ourselves in zebrafish. However, if you would be willing to test it in zebrafish and give us your results whether positive or negative, I can offer you a testing discount.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product in zebrafish.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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Answer

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

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