Key features and details
- Rabbit polyclonal to Hes1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Hes1 antibody
See all Hes1 primary antibodies
DescriptionRabbit polyclonal to Hes1
SpecificityThis antibody reacts specifically with mouse 30 kDa Hes1 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
Concentration information loading...
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab71559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 30 kDa.Can be blocked with Hes1 peptide (ab126460).|
FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
- Information by UniProt
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
Anti-Hes1 antibody (ab71559) at 1/2000 dilution + MCF-7 at 20 µg
HRP conjugated goat anti-rabbit at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Exposure time: 15 seconds
Blocked with 5% milk at RT
All lanes : Anti-Hes1 antibody (ab71559)
Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Predicted band size: 30 kDa
Actin blot given as control.
ab71559 has been referenced in 75 publications.
- Venugopal N et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
- Pan W et al. TSP2 acts as a suppresser of cell invasion, migration and angiogenesis in medulloblastoma by inhibiting the Notch signaling pathway. Brain Res 1718:223-230 (2019). PubMed: 31063715
- Jiandong L et al. Trichosanthes kirilowii lectin ameliorates streptozocin-induced kidney injury via modulation of the balance between M1/M2 phenotype macrophage. Biomed Pharmacother 109:93-102 (2019). PubMed: 30396096
- Chen FZ et al. MicroRNA-98 reduces amyloid ß-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice. Int J Mol Med 43:91-102 (2019). PubMed: 30365070
- Wang XF et al. DLX5 gene regulates the Notch signaling pathway to promote glomerulosclerosis and interstitial fibrosis in uremic rats. J Cell Physiol 234:21825-21837 (2019). PubMed: 31297803