Anti-Hes1 antibody (ab71559)
Key features and details
- Rabbit polyclonal to Hes1
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Hes1 antibody
See all Hes1 primary antibodies -
Description
Rabbit polyclonal to Hes1 -
Host species
Rabbit -
Specificity
This antibody reacts specifically with mouse 30 kDa Hes1 protein. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Hes1 (C terminal).
Database link: Q14469 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
ICC/IF | (4) |
Use at an assay dependent concentration.
|
IHC-P | (5) |
Use at an assay dependent concentration.
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WB | (14) |
1/200 - 1/2000. Predicted molecular weight: 30 kDa.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/200 - 1/2000. Predicted molecular weight: 30 kDa. |
Target
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Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Images
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Anti-Hes1 antibody (ab71559) at 1/2000 dilution + MCF-7 at 20 µg
Secondary
HRP conjugated goat anti-rabbit at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Exposure time: 15 secondsBlocked with 5% milk at RT
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All lanes : Anti-Hes1 antibody (ab71559)
Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Predicted band size: 30 kDa
Actin blot given as control.
Datasheets and documents
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SDS download
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Datasheet download
References (126)
ab71559 has been referenced in 126 publications.
- Akdemir ES et al. Lunatic Fringe-GFP Marks Lamina-Specific Astrocytes That Regulate Sensory Processing. J Neurosci 42:567-580 (2022). PubMed: 34872929
- Silva E et al. Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos. Methods Mol Biol 2472:173-185 (2022). PubMed: 35674900
- Ni T et al. Medial prefrontal cortex Notch1 signalling mediates methamphetamine-induced psychosis via Hes1-dependent suppression of GABAB1 receptor expression. Mol Psychiatry 27:4009-4022 (2022). PubMed: 35732696
- Shi Y et al. Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling. Int J Biol Sci 18:4233-4244 (2022). PubMed: 35844785
- Luan Y et al. REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway. J Cell Biochem 122:16-28 (2021). PubMed: 32965043