Key features and details
- Rabbit polyclonal to Hes1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Hes1 antibody
See all Hes1 primary antibodies
DescriptionRabbit polyclonal to Hes1
SpecificityThis antibody reacts specifically with mouse 30 kDa Hes1 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
Concentration information loading...
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab71559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 30 kDa.Can be blocked with Hes1 peptide (ab126460).|
FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
- Information by UniProt
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
Anti-Hes1 antibody (ab71559) at 1/2000 dilution + MCF-7 at 20 µg
HRP conjugated goat anti-rabbit at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Exposure time: 15 seconds
Blocked with 5% milk at RT
All lanes : Anti-Hes1 antibody (ab71559)
Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Predicted band size: 30 kDa
Actin blot given as control.
ab71559 has been referenced in 82 publications.
- Venugopal N et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
- Xia P et al. lncRNA CEBPA-AS1 Overexpression Inhibits Proliferation and Migration and Stimulates Apoptosis of OS Cells via Notch Signaling. Mol Ther Nucleic Acids 19:1470-1481 (2020). PubMed: 32160715
- Morrugares R et al. Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2. Cell Mol Life Sci 77:2621-2639 (2020). PubMed: 31605148
- Zhang Z et al. GANT61 and Valproic Acid Synergistically Inhibited Multiple Myeloma Cell Proliferation via Hedgehog Signaling Pathway. Med Sci Monit 26:e920541 (2020). PubMed: 32054823
- Wei B et al. MicroRNA-145-5p attenuates high glucose-induced apoptosis by targeting the Notch signaling pathway in podocytes. Exp Ther Med 19:1915-1924 (2020). PubMed: 32104249