Recombinant
RabMAb

Recombinant Anti-Hes1 antibody [EPR4226] (ab108937)

Rabbit recombinant monoclonal Hes1 antibody [EPR4226]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 23 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-Hes1 antibody [EPR4226]
    See all Hes1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4226] to Hes1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Hes1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Fetal brain, PC12, and SH-SY5Y cell lysates. Human placenta tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/40 - 1/100.

 

WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.

 

IHC-P 1/90 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt 1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.
    • Domain

      Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
      The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
      The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLHb39 antibody
      • C-HAIRY1 antibody
      • c-hairy1A antibody
      • Class B basic helix-loop-helix protein 39 antibody
      • FLJ20408 antibody
      • Hairy and enhancer of split 1 (Drosophila) antibody
      • Hairy and enhancer of split 1 antibody
      • Hairy homolog (Drosophila) antibody
      • Hairy homolog antibody
      • Hairy like antibody
      • Hairy, Drosophila, homolog of antibody
      • Hairy-like protein antibody
      • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
      • HAIRY1 antibody
      • HES-1 antibody
      • hes1 antibody
      • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
      • HES1_HUMAN antibody
      • HHL antibody
      • HL antibody
      • HRY antibody
      • MGC129109 antibody
      • OTTHUMP00000209031 antibody
      • RHL antibody
      • Transcription factor HES-1 antibody
      see all

    Images

    • Anti-Hes1 antibody [EPR4226] (ab108937) at 1/1000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Primary ab concentration dilution: 1:200, (0.5ug/ml), Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG, Secondary ab concentration: Prediluted, Tissue: Human brain, Fixative: Paraffin-embedded sections, Counter stain: Hematoxylin Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9

    • Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab108937 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    • Overlay histogram showing SH-SY5Y cells stained with unpurified ab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108937, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • All lanes : Anti-Hes1 antibody [EPR4226] (ab108937) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 30 kDa

    • Immunohistochemical staining of Hes1 in paraffin-embedded human placenta tissue with unpurified ab108937 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical staining of paraffin embedded human brain with unpurified ab108937 at a working dilution of 1 in 90. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab108937 at a dilution of 1/40. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    References

    This product has been referenced in:

    • Liu W  et al. Mesenchymal stem cells alleviate the early brain injury of subarachnoid hemorrhage partly by suppression of Notch1-dependent neuroinflammation: involvement of Botch. J Neuroinflammation 16:8 (2019). Read more (PubMed: 30646897) »
    • Cui Q  et al. GXYLT2 accelerates cell growth and migration by regulating the Notch pathway in human cancer cells. Exp Cell Res 376:1-10 (2019). Read more (PubMed: 30716301) »
    See all 33 Publications for this product

    Customer reviews and Q&As

    1-10 of 10 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E14.5 Kidney)
    Permeabilization
    Yes - Triton X 100
    Specification
    E14.5 Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 07 2017

    Answer

    Thank you very much for your call today and for letting us know about the trouble with this antibody.

    Your credit note ID is ***

    I am sorry that this antibody did notwork as expected.I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    Please let me know ifthere is anything else that we can do for you. Have a nice weekend!

    Read More

    Question
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Question

    Catalog number(s): 108937
    Product(s): Rabbit monoclonal [EPR4226] to Hes1
    -----------------------------------

    Telephone section
    Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
    A. Yes
    Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
    A. I do not remember

    Protocol advice section
    Q. Did Abcam's Scientific Support give you protocol advice?
    A.Yes
    Q. How did you feel about being given protocol advice?
    A.I was not expecting to be given protocol advice, but was happy to receive this
    Q. Did the product work successfully after following the advice?
    A.No
    Q. Why have you not tried the protocol advice? (Select all that apply.)
    A.

    Free of charge replacement section
    Q. Did Abcam's Scientific Support give you a free of charge replacement?
    A. Yes

    Q. Did the replacement product work successfully?
    A.Yes
    Credit note / refund section
    Q. Did Abcam's Scientific Support give you a credit note or a refund?
    A.No

    Q. Would you have preferred a different outcome from receiving a credit note or refund?
    A.
    Overall satisfaction section
    Q. How satisfied are you with how the complaint was handled?
    A.Satisfied
    Q. Could we have done anything to better resolve the problem?
    A.More timely response to product issues when problems arise with products used in proven/time-tested assays.
    Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
    A.No, I would not mind
    Q. Please provide a phone number or email address where we can reach you.
    A.
    Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
    A.

    Read More
    Answer

    Thank you for your participation in Abcam's post complaint survey.

    If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

    Read More

    Question
    Answer

    I have issued a free of charge replacement for one vialof ab108937.


    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Answer

    Thanks for your reply.

    Unfortunately neither of these numers correspond to an order from Sapphire for a vial of ab55265. Please check the Abcam order referenc number or your internal PO number. Also it would be helpful to know when the order was placed as well.

    Thanks for the additional information.

    Read More

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab71559.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Answer

    Thank you for contacting us.
    I am sorry to hear that the antibody is not working as expected.

    Could you please send me the following protocol information so that we can evaluate the problem better:

    1) Was the weak signal specific or rather only background?
    2) What antibody dilution and incubation time was used?
    3) What was used for blocking?
    4) Which antigen retrieval method was employed?
    5) What detection system was used and does it work well with other primary antibodies?

    For a free of charge replacement, the following antibodies have been tsted in IHC-P and human samples: ab71559, ab49170, ab119776 and ab87395. Please let me also know which one of these antibodies your colleague might be interested as a replacement.

    I look forward to hear back from you and to assist you in resolving this case.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thanks for your enquiry.

    Thanks for submitting your completed WB questionnaire and for including the blotting data. It looks like you have done a good job of trouble shooting.

    About the cell lysate samples, are these samples which are known to express detectable levels of Hes1?

    And regarding the pure recombinant Hes1 protein;
    1. Is that full lenght protein?
    2. Is it based on the human sequence of Hes1?

    Thanks in advance for the additional information.

    Read More

    Answer

    Thank you for your enquiry.

    I am sorry but it is not known which specific cell type is being stained in the placental tissue.

    Please contact us again if you have any further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up