Recombinant
RabMAb

Recombinant Anti-Hes1 antibody [EPR4226] (Alexa Fluor® 647) (ab196577)

Overview

  • Product name
    Anti-Hes1 antibody [EPR4226] (Alexa Fluor® 647)
    See all Hes1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4226] to Hes1 (Alexa Fluor® 647)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Hes1 aa 200 to the C-terminus.

  • Positive control
    • ICC/IF: SK-N-SH cells. Flow Cyt: SHSY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/200.

This product gave a positive signal in SK-N-SH cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
    The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb39 antibody
    • C-HAIRY1 antibody
    • c-hairy1A antibody
    • Class B basic helix-loop-helix protein 39 antibody
    • FLJ20408 antibody
    • Hairy and enhancer of split 1 (Drosophila) antibody
    • Hairy and enhancer of split 1 antibody
    • Hairy homolog (Drosophila) antibody
    • Hairy homolog antibody
    • Hairy like antibody
    • Hairy, Drosophila, homolog of antibody
    • Hairy-like protein antibody
    • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
    • HAIRY1 antibody
    • HES-1 antibody
    • hes1 antibody
    • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
    • HES1_HUMAN antibody
    • HHL antibody
    • HL antibody
    • HRY antibody
    • MGC129109 antibody
    • OTTHUMP00000209031 antibody
    • RHL antibody
    • Transcription factor HES-1 antibody
    see all

Images

  • ab196577 staining Hes1 in SK-N-SH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196577 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    This product also gave a positive signal under the same testing conditions in SK-N-SH cells fixed with 100% methanol (5min).

References

This product has been referenced in:
  • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). Read more (PubMed: 29993362) »
  • Zheng G  et al. Chronic stress and intestinal barrier dysfunction: Glucocorticoid receptor and transcription repressor HES1 regulate tight junction protein Claudin-1 promoter. Sci Rep 7:4502 (2017). Read more (PubMed: 28674421) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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