Recombinant
RabMAb

Recombinant Anti-Hes1 antibody [EPR4226] - BSA and Azide free (ab221788)

Overview

  • Product name

    Anti-Hes1 antibody [EPR4226] - BSA and Azide free
    See all Hes1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4226] to Hes1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Hes1 aa 200-300.

  • Positive control

    • Fetal brain, PC12, and SH-SY5Y cell lysates. Human placenta tissue.
  • General notes

    Ab221788 is the carrier-free version of ab108937. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab221788 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab221788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.

 

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.
    • Domain

      Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
      The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
      The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLHb39 antibody
      • C-HAIRY1 antibody
      • c-hairy1A antibody
      • Class B basic helix-loop-helix protein 39 antibody
      • FLJ20408 antibody
      • Hairy and enhancer of split 1 (Drosophila) antibody
      • Hairy and enhancer of split 1 antibody
      • Hairy homolog (Drosophila) antibody
      • Hairy homolog antibody
      • Hairy like antibody
      • Hairy, Drosophila, homolog of antibody
      • Hairy-like protein antibody
      • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
      • HAIRY1 antibody
      • HES-1 antibody
      • hes1 antibody
      • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
      • HES1_HUMAN antibody
      • HHL antibody
      • HL antibody
      • HRY antibody
      • MGC129109 antibody
      • OTTHUMP00000209031 antibody
      • RHL antibody
      • Transcription factor HES-1 antibody
      see all

    Images

    • Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab108937 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

    • Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab108937 at a dilution of 1/40. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

    • Primary ab concentration dilution: 1:200, (0.5ug/ml), Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG, Secondary ab concentration: Prediluted, Tissue: Human brain, Fixative: Paraffin-embedded sections, Counter stain: Hematoxylin Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

    • Immunohistochemical staining of paraffin embedded human brain with unpurified ab108937 at a working dilution of 1 in 90. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

    • Immunohistochemical staining of Hes1 in paraffin-embedded human placenta tissue with unpurified ab108937 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Overlay histogram showing SH-SY5Y cells stained with unpurified ab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108937, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108937).

    References

    This product has been referenced in:

    • Zhou Q  et al. DACH1 suppresses epithelial to mesenchymal transition (EMT) through Notch1 pathway and reverses progestin resistance in endometrial carcinoma. Cancer Med 8:4380-4388 (2019). Read more (PubMed: 31215145) »
    • Xie F  et al. Vaccarin attenuates the human EA.hy926 endothelial cell oxidative stress injury through inhibition of Notch signaling. Int J Mol Med 35:135-42 (2015). Human . Read more (PubMed: 25352009) »
    See all 6 Publications for this product

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