Product nameAnti-HES5 antibody
See all HES5 primary antibodies
DescriptionRabbit polyclonal to HES5
SpecificityDetects endogenous levels of HES5 protein. Not tested with other proteins.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide, corresponding to internal sequence amino acids of Human HES5.
- Human brain, cerebellum tissue; Rat and Mouse brain lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 99% Tris glycine, 0.5% BSA
Tris glycine (without Mg2+, Ca2+)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 18 kDa.|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription.
Tissue specificityExpressed in fetal heart and brain tumors.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
- Information by UniProt
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cerebellum tissue labelling HES5 with ab133424 antibody at 2.5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
All lanes : Anti-HES5 antibody (ab133424) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Predicted band size: 18 kDa
ab133424 has been referenced in 1 publication.
- Xu ZX et al. Expression changes of the notch signaling pathway of PC12 cells after oxygen glucose deprivation. Int J Biol Macromol 118:1984-1988 (2018). PubMed: 30017986