Overview

  • Product name
  • Description
    Rabbit polyclonal to HES5
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of HES5 protein. Not tested with other proteins.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids of Human HES5.

  • Positive control
    • Human brain, cerebellum tissue; Rat and Mouse brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab133424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/10000.

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificity
    Expressed in fetal heart and brain tumors.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cerebellum tissue labelling HES5 with ab133424 antibody at 2.5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-HES5 antibody (ab133424) at 1/500 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Predicted band size: 18 kDa

References

This product has been referenced in:
  • Xu ZX  et al. Expression changes of the notch signaling pathway of PC12 cells after oxygen glucose deprivation. Int J Biol Macromol 118:1984-1988 (2018). Read more (PubMed: 30017986) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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