Anti-HES5 antibody (ab221598)
Key features and details
- Rabbit polyclonal to HES5
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HES5 antibody
See all HES5 primary antibodies -
Description
Rabbit polyclonal to HES5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human HES5 aa 1-100.
Database link: Q5TA89 -
Positive control
- ICC/IF: HaCaT cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. -
Tissue specificity
Expressed in fetal heart and brain tumors. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
see all -
Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HES5 antibody (ab221598)
PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for HES5 (green) using ab221598 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221598 has not yet been referenced specifically in any publications.