Overview

  • Product name
  • Description
    Rabbit polyclonal to HES5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human HES5 aa 3-30.
    Sequence:

    PSTVAVELLSPKEKNRLRKPVVEKMRRD


    Database link: Q5TA89

  • Positive control
    • ICC/IF: HaCaT cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab221598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificity
    Expressed in fetal heart and brain tumors.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for HES5 (green) using ab221598 at 4 µg/ml in ICC/IF.

References

ab221598 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221598.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up