Anti-HES5 antibody (ab221598)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-HES5 antibody
See all HES5 primary antibodies -
DescriptionRabbit polyclonal to HES5
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human HES5 aa 3-30.
Sequence:PSTVAVELLSPKEKNRLRKPVVEKMRRD
Database link: Q5TA89 -
Positive control
- ICC/IF: HaCaT cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab221598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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FunctionTranscriptional repressor of genes that require a bHLH protein for their transcription.
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Tissue specificityExpressed in fetal heart and brain tumors.
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Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
DomainHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
see all -
Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HES5 antibody (ab221598)PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for HES5 (green) using ab221598 at 4 µg/ml in ICC/IF.
Datasheets and documents
References
ab221598 has not yet been referenced specifically in any publications.
