Anti-HES5 antibody (ab25374)
Key features and details
- Rabbit polyclonal to HES5
- Suitable for: WB, ELISA, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HES5 antibody
See all HES5 primary antibodies -
Description
Rabbit polyclonal to HES5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse HES5 aa 50-150.
Database link: P70120 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituents: PBS, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-Fr | (1) |
1/200 - 1/1000.
This product has not been tested with paraffin sections. |
Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
1/200 - 1/1000. This product has not been tested with paraffin sections. |
Target
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Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. -
Tissue specificity
Expressed in fetal heart and brain tumors. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
see all -
Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
see all
Images
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ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr. The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes. A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes. HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab25374 has been referenced in 19 publications.
- Du Z et al. SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway. Biomed Res Int 2022:9235837 (2022). PubMed: 36246971
- Gao Q et al. Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia. Neural Regen Res 16:1252-1257 (2021). PubMed: 33318402
- Morrugares R et al. Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2. Cell Mol Life Sci 77:2621-2639 (2020). PubMed: 31605148
- Fan J et al. Targeting the Notch and TGF-ß signaling pathways to prevent retinal fibrosis in vitro and in vivo. Theranostics 10:7956-7973 (2020). PubMed: 32724452
- Huang H et al. M1 Macrophage Activated by Notch Signal Pathway Contributed to Ventilator-Induced Lung Injury in Chronic Obstructive Pulmonary Disease Model. J Surg Res 244:358-367 (2019). PubMed: 31323391