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Ab25374-this is an ab against nuclear protein. On the datasheet, in the IF staining figure, it seems cytopasmic. Please check and advise.
Asked on Jan 19 2010
The Uniprot localization of Hes5 is listed as Nuclear (by similarity) to other transcriptional repressors. However, Hes5 is widely expressed in the developing nervous system (Akazawa et al., 1992) and in embryos Hes5 is ubiquitously expressed inside the CNS, especially in undifferentiated cells (http://www.gensat.org/imagenavigator.jsp?imageID=15026). The image shows IF of e14.5 mouse embryo and displays both nuclear and cytoplasmic staining for ab25374. I hope this clarifies the matter.
Answered on Jan 19 2010