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Dear Abcam technical service,
I would like to ask to you some detailed information about your anti HES5 antibody(ab25374).
I've read that it has been tested for Western blot.
Do you have the protocol for Western blot that you've used for testing it?
Thank you in advance
Asked on Jun 15 2012
Thank you for your inquiry.
I am happy to confirm that ab25374 is tested and guaranteed for WB with mouse and human samples.
It was tested using standard WB procedure which can be found here:
I also would like to suggest to use a positive control like mouse embryonic cortex.
I hope this information is helpful and wish you a good weekend.
Answered on Jun 15 2012