Anti-HES5 antibody [EPR15578] Recombinant (ab194111)

Product name

Anti-HES5 antibody [EPR15578]
See all HES5 primary antibodies
Description

Rabbit monoclonal [EPR15578] to HES5
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human HES5 aa 1-150. The exact sequence is proprietary.
Database link: Q5TA89

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Positive control
- 293, C6 and PC-12 cell lysates; Human cerebellum, Human fetal brain, mouse brain, mouse kidney, mouse spleen, rat brain, rat kidney and rat spleen lysates; Human glioblastoma, Human adenocarcinoma of endometrium and mouse cerebral cortex tissues.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15578
Isotype

IgG
Research areas

Alternative Versions
- Anti-HES5 antibody [EPR15578] - BSA and Azide free (ab236142)
Positive Controls

Our Abpromise guarantee covers the use of ab194111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/500 - 1/1000.
WB		1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP		1/40.

Function

Transcriptional repressor of genes that require a bHLH protein for their transcription.
Tissue specificity

Expressed in fetal heart and brain tumors.
Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q5TA89 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
- Unigene: 137268 Mouse
- Unigene: 22422 Rat
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Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
- Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
- HES 5 antibody
- hes family bHLH transcription factor 5 antibody
- Hes5 antibody
- HES5 protein antibody
- HES5_HUMAN antibody
- MGI:104876 antibody
- Transcription factor HES-5 antibody
see all

Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + 293 cell lysate at 10 µg

Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + Human cerebellum lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

All lanes : Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 cell lysate
Lane 8 : PC-12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)

Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.

Datasheet

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

This product has been referenced in:

Arai MA et al. The Notch inhibitor cowanin accelerates nicastrin degradation. Sci Rep 8:5376 (2018). Read more (PubMed: 29599482) »
Hu S et al. The function of Notch1 intracellular domain in the differentiation of gastric cancer. Oncol Lett 15:6171-6178 (2018). Read more (PubMed: 29616098) »

See all 6 Publications for this product

Publishing research using ab194111? Please let us know so that we can cite the reference in this datasheet.

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Anti-HES5 antibody [EPR15578] (ab194111)

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