Recombinant Anti-HES5 antibody [EPR15578] (ab194111)

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Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15578] to HES5
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-HES5 antibody [EPR15578]
See all HES5 primary antibodies
Description

Rabbit monoclonal [EPR15578] to HES5
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293, C6 and PC-12 cell lysates; Human cerebellum, Human fetal brain, mouse brain, mouse kidney, mouse spleen, rat brain, rat kidney and rat spleen lysates; Human glioblastoma, Human adenocarcinoma of endometrium and mouse cerebral cortex tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15578
Isotype

IgG
Research areas

Alternative Versions
- Anti-HES5 antibody [EPR15578] - BSA and Azide free (ab236142)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP		1/40.

Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP 1/40.

Function

Transcriptional repressor of genes that require a bHLH protein for their transcription.
Tissue specificity

Expressed in fetal heart and brain tumors.
Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q5TA89 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 388585 Human
- Entrez Gene: 15208 Mouse
- Entrez Gene: 79225 Rat
- Omim: 607348 Human
- SwissProt: Q5TA89 Human
- SwissProt: P70120 Mouse
- SwissProt: Q03062 Rat
- Unigene: 57971 Human
- Unigene: 137268 Mouse
- Unigene: 22422 Rat
see all
Alternative names
- bHLHb38 antibody
- Class B basic helix-loop-helix protein 38 antibody
- Hairy and enhancer of split 5 antibody
- Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
- HES 5 antibody
- hes family bHLH transcription factor 5 antibody
- Hes5 antibody
- HES5 protein antibody
- HES5_HUMAN antibody
- MGI:104876 antibody
- Transcription factor HES-5 antibody
see all

Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + 293 cell lysate at 10 µg

Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + Human cerebellum lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution + Human fetal brain lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HES5 antibody [EPR15578] (ab194111)

All lanes : Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 cell lysate
Lane 8 : PC-12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa
Observed band size: 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)

Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HES5 antibody [EPR15578] (ab194111)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab194111? Please let us know so that we can cite the reference in this datasheet.

ab194111 has been referenced in 13 publications.

Chen FZ et al. MicroRNA-98 reduces amyloid ß-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice. Int J Mol Med 43:91-102 (2019). PubMed: 30365070
Peng Z et al. Inhibition of Notch1 signaling promotes neuronal differentiation and improves functional recovery in spinal cord injury through suppressing the activation of Ras homolog family member A. J Neurochem 150:709-722 (2019). PubMed: 31339573
Wang XF et al. DLX5 gene regulates the Notch signaling pathway to promote glomerulosclerosis and interstitial fibrosis in uremic rats. J Cell Physiol 234:21825-21837 (2019). PubMed: 31297803
Manning CS et al. Quantitative single-cell live imaging links HES5 dynamics with cell-state and fate in murine neurogenesis. Nat Commun 10:2835 (2019). PubMed: 31249377
Xu H et al. NFIX Circular RNA Promotes Glioma Progression by Regulating miR-34a-5p via Notch Signaling Pathway. Front Mol Neurosci 11:225 (2018). PubMed: 30072869

View all Publications for this product

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (13)

Customer reviews and Q&As