Recombinant
RabMAb

Recombinant Anti-HES5 antibody [EPR15578] (ab194111)

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Overview

  • Product name

    Anti-HES5 antibody [EPR15578]
    See all HES5 primary antibodies

  • Description

    Rabbit monoclonal [EPR15578] to HES5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human HES5 aa 1-150. The exact sequence is proprietary.
    Database link: Q5TA89

  • Positive control

    • 293, C6 and PC-12 cell lysates; Human cerebellum, Human fetal brain, mouse brain, mouse kidney, mouse spleen, rat brain, rat kidney and rat spleen lysates; Human glioblastoma, Human adenocarcinoma of endometrium and mouse cerebral cortex tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab194111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP 1/40.

Target

  • Function

    Transcriptional repressor of genes that require a bHLH protein for their transcription.

  • Tissue specificity

    Expressed in fetal heart and brain tumors.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.

  • Domain

    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Images

  • Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + 293 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    Western blot - Anti-HES5 antibody [EPR15578] (ab194111)
    All lanes : Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : C6 cell lysate
    Lane 8 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

    Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

    Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HES5 antibody [EPR15578] (ab194111)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)
    Immunoprecipitation - Anti-HES5 antibody [EPR15578] (ab194111)

    Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Chen FZ  et al. MicroRNA-98 reduces amyloid ß-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice. Int J Mol Med 43:91-102 (2019). Read more (PubMed: 30365070) »
  • Peng Z  et al. Inhibition of Notch1 signaling promotes neuronal differentiation and improves functional recovery in spinal cord injury through suppressing the activation of Ras homolog family member A. J Neurochem 150:709-722 (2019). Read more (PubMed: 31339573) »
See all 13 Publications for this product

Publishing research using ab194111? Please let us know so that we can cite the reference in this datasheet.

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