Recombinant
RabMAb

Anti-HES5 antibody [EPR15578] - BSA and Azide free (ab236142)

Overview

  • Product name
    Anti-HES5 antibody [EPR15578] - BSA and Azide free
    See all HES5 primary antibodies
  • Description
    Rabbit monoclonal [EPR15578] to HES5 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human HES5 aa 1-150. The exact sequence is proprietary.
    Database link: Q5TA89

  • Positive control
    • IHC-P: Human glioblastoma tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab236142 is a PBS-only buffer format of ab194111. Please refer to ab194111 for recommended dilutions, protocols, and image data. 

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function
    Transcriptional repressor of genes that require a bHLH protein for their transcription.
  • Tissue specificity
    Expressed in fetal heart and brain tumors.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Domain
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Images

  • Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194111).

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194111).

  • Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194111).

  • Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194111).

References

ab236142 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236142.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up