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    hes6-antibody-ab172800.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH
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Anti-HES6 antibody (ab172800)

  • Datasheet
  • SDS
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Western blot - Anti-HES6 antibody (ab172800)

    Key features and details

    • Mouse polyclonal to HES6
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human HES6 protein (ab132389)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-HES6 antibody
      See all HES6 primary antibodies
    • Description

      Mouse polyclonal to HES6
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Full length protein corresponding to Human HES6 aa 1-224.
      Sequence:

      MAPPAAPGRDRVGREDEDGWETRGDRKARKPLVEKKRRARINESLQELRL LLAGAEVQAKLENAEVLELTVRRVQGVLRGRAREREQLQAEASERFAAGY IQCMHEVHTFVSTCQAIDATVAAELLNHLLESMPLREGSSFQDLLGDALA GPPRAPGRSGWPAGGAPGSPIPSPPGPGDDLCSDLEEAPEAELSQAPAEG PDLVPAALGSLTTAQIARSVWRPW


      Database link: NP_061115.2
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • HES6 transfected 293T cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.4
      Constituent: 100% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • HLH
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Co-factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant Human HES6 protein (ab132389)
    • Related Products

      • Recombinant Human HES6 protein (ab132389)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab172800 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.

    Target

    • Relevance

      HES6 is a transcription cofactor that iteself does not bind to DNA but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. It also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCFEA2/E47, allowing ASCL1 and TCFEA2 to upregulate transcription in its presence. HES6 appears to have novel functions in tumour and cancer cell lines, although these have not yet been elucidated fully.
    • Cellular localization

      Nuclear
    • Database links

      • Entrez Gene: 55502 Human
      • Entrez Gene: 55927 Mouse
      • Omim: 610331 Human
      • SwissProt: Q96HZ4 Human
      • SwissProt: Q9JHE6 Mouse
      • Unigene: 42949 Human
      • Unigene: 280029 Mouse
      • Alternative names

        • bHLHb41 antibody
        • bHLHc23 antibody
        • C HAIRY1 antibody
        • Class B basic helix-loop-helix protein 41 antibody
        • Hairy and enhancer of split 6 antibody
        • Hairy/enhancer of split 6, Drosophila, homolog of, 6 antibody
        • HES 6 antibody
        • hes family bHLH transcription factor 6 antibody
        • Transcription cofactor HES6 antibody
        see all

      Images

      • Western blot - Anti-HES6 antibody (ab172800)
        Western blot - Anti-HES6 antibody (ab172800)
        All lanes : Anti-HES6 antibody (ab172800) at 1 µg/ml

        Lane 1 : HES6 transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 15 µl per lane.

        Developed using the ECL technique.

        Predicted band size: 24 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab172800? Please let us know so that we can cite the reference in this datasheet.

      ab172800 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-HES6 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Mouse Liver)
      Gel Running Conditions
      Reduced Denaturing (12)
      Loading amount
      35 µg
      Specification
      Mouse Liver
      Blocking step
      Odyssey Blocking Buffer (TBS) 1:1 in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
      Read More

      Thomas Gantert

      Verified customer

      Submitted Aug 31 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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