Overview

  • Product name
    Anti-HES6 antibody
    See all HES6 primary antibodies
  • Description
    Mouse polyclonal to HES6
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length protein corresponding to Human HES6 aa 1-224.
    Sequence:

    MAPPAAPGRDRVGREDEDGWETRGDRKARKPLVEKKRRARINESLQELRL LLAGAEVQAKLENAEVLELTVRRVQGVLRGRAREREQLQAEASERFAAGY IQCMHEVHTFVSTCQAIDATVAAELLNHLLESMPLREGSSFQDLLGDALA GPPRAPGRSGWPAGGAPGSPIPSPPGPGDDLCSDLEEAPEAELSQAPAEG PDLVPAALGSLTTAQIARSVWRPW


    Database link: NP_061115.2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HES6 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab172800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.

Target

  • Relevance
    HES6 is a transcription cofactor that iteself does not bind to DNA but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. It also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCFEA2/E47, allowing ASCL1 and TCFEA2 to upregulate transcription in its presence. HES6 appears to have novel functions in tumour and cancer cell lines, although these have not yet been elucidated fully.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • bHLHb41 antibody
      • bHLHc23 antibody
      • C HAIRY1 antibody
      • Class B basic helix-loop-helix protein 41 antibody
      • Hairy and enhancer of split 6 antibody
      • Hairy/enhancer of split 6, Drosophila, homolog of, 6 antibody
      • HES 6 antibody
      • hes family bHLH transcription factor 6 antibody
      • Transcription cofactor HES6 antibody
      see all

    Images

    • Western blot - Anti-HES6 antibody (ab172800)
      Western blot - Anti-HES6 antibody (ab172800)
      All lanes : Anti-HES6 antibody (ab172800) at 1 µg/ml

      Lane 1 : HES6 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Developed using the ECL technique.

      Predicted band size: 24 kDa

    References

    ab172800 has not yet been referenced specifically in any publications.

    Publishing research using ab172800? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-HES6 antibody

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse Liver)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    35 µg
    Specification
    Mouse Liver
    Blocking step
    Odyssey Blocking Buffer (TBS) 1:1 in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More
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    Username

    Thomas Gantert

    Verified customer

    Submitted Aug 31 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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