Key features and details
- Mouse polyclonal to HESX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).|
RelevanceHomeobox protein expressed in embryonic stem cells and targetd by Sox2, Oct4 and Nanog. Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor.
- ANF antibody
- CPHD5 antibody
- hANF antibody
All lanes : Anti-HESX1 antibody (ab67728) at 1/500 dilution
Lane 1 : HESX1 transfected lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
ab67728 has been referenced in 1 publication.
- Takagi M et al. A novel mutation in HESX1 causes combined pituitary hormone deficiency without septo optic dysplasia phenotypes. Endocr J 63:405-10 (2016). PubMed: 26781211